Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5′ untranslated region and participates in virus replication

Jing Yi Lin, Mei Ling Li, Peng Nien Huang, Kun Yi Chien, Jim Tong Horng, Shin Ru Shih

研究成果: 雜誌貢獻文章

83 引文 (Scopus)

摘要

Enterovirus 71 (EV71) is a picornavirus that can cause severe neurological complications in children. Like other picornaviruses, the genomic RNA of EV71 contains a long 59 untranslated region (UTR). Cellular proteins interact with the EV71 5′ UTR, and these interactions are important for virus replication. Using an RNA pull-down assay and proteomics approaches, this study identified the heterogeneous nuclear ribonucleoprotein K (hnRNP K) as one of the EV71 5′ UTR-associated proteins. The interaction between hnRNP K and the 5′ UTR was further confirmed by mapping the interaction regions to stem-loops I-II and IV in the 5′ UTR. During EV71 infection, hnRNP K was enriched in the cytoplasm where virus replication occurs, whereas hnRNP K was localized in the nucleus in mock-infected cells. Viral yields were found to be significantly lower in hnRNP K knockdown cells and viral RNA synthesis was delayed in hnRNP K knockdown cells in comparison with negative-control cells treated with small interfering RNA. These results suggest that hnRNP K interacts with the EV71 5′ UTR and participates in virus replication.

原文英語
頁(從 - 到)2540-2549
頁數10
期刊Journal of General Virology
89
發行號10
DOIs
出版狀態已發佈 - 2008
對外發佈Yes

指紋

Heterogeneous-Nuclear Ribonucleoprotein K
Enterovirus
5' Untranslated Regions
Virus Replication
Nuclear Proteins
Picornaviridae
Enterovirus Infections
RNA
Untranslated Regions
Viral RNA
protein K
Proteomics
Small Interfering RNA
Cytoplasm
Proteins

ASJC Scopus subject areas

  • Virology

引用此文

Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5′ untranslated region and participates in virus replication. / Lin, Jing Yi; Li, Mei Ling; Huang, Peng Nien; Chien, Kun Yi; Horng, Jim Tong; Shih, Shin Ru.

於: Journal of General Virology, 卷 89, 編號 10, 2008, p. 2540-2549.

研究成果: 雜誌貢獻文章

Lin, Jing Yi ; Li, Mei Ling ; Huang, Peng Nien ; Chien, Kun Yi ; Horng, Jim Tong ; Shih, Shin Ru. / Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5′ untranslated region and participates in virus replication. 於: Journal of General Virology. 2008 ; 卷 89, 編號 10. 頁 2540-2549.
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abstract = "Enterovirus 71 (EV71) is a picornavirus that can cause severe neurological complications in children. Like other picornaviruses, the genomic RNA of EV71 contains a long 59 untranslated region (UTR). Cellular proteins interact with the EV71 5′ UTR, and these interactions are important for virus replication. Using an RNA pull-down assay and proteomics approaches, this study identified the heterogeneous nuclear ribonucleoprotein K (hnRNP K) as one of the EV71 5′ UTR-associated proteins. The interaction between hnRNP K and the 5′ UTR was further confirmed by mapping the interaction regions to stem-loops I-II and IV in the 5′ UTR. During EV71 infection, hnRNP K was enriched in the cytoplasm where virus replication occurs, whereas hnRNP K was localized in the nucleus in mock-infected cells. Viral yields were found to be significantly lower in hnRNP K knockdown cells and viral RNA synthesis was delayed in hnRNP K knockdown cells in comparison with negative-control cells treated with small interfering RNA. These results suggest that hnRNP K interacts with the EV71 5′ UTR and participates in virus replication.",
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AU - Horng, Jim Tong

AU - Shih, Shin Ru

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