Heterodimerization of opioid receptor-like 1 and μ-opioid receptors impairs the potency of μ receptor agonist

Hung Li Wang, Chia Yu Hsu, Pei Chen Huang, Yo Li Kuo, Allen Hon Lun Li, Tu Hsueh Yeh, An Swe Tso, Ying Ling Chen

研究成果: 雜誌貢獻文章同行評審

62 引文 斯高帕斯(Scopus)

摘要

Nociceptin activation of ORL1 (opioid receptor-like 1 receptor) has been shown to antagonize μ receptor-mediated analgesia at the supraspinal level. ORL1 and μ-opioid receptor (μR) are co-expressed in several subpopulations of CNS neurons involved in regulating pain transmission. The amino acid sequence of ORL1 also shares a high degree of homology with that of μ receptor. Thus, it is hypothesized that ORL1 and μR interact to form the heterodimer and that ORL1/μR heterodimerization may be one molecular basis for ORL1-mediated antiopioid effects in the brain. To test this hypothesis, myc-tagged ORL1 and HA-tagged μR are co-expressed in human embryonic kidney (HEK) 293 cells. Co-immunoprecipitation experiments demonstrate that ORL1 dimerizes with μR and that intracellular C-terminal tails of ORL1 and μR are required for the formation of ORL1/μR heterodimer. Second messenger assays further indicate that formation of ORL1/μR heterodimer selectively induces cross-desensitization of μR and impairs the potency by which [D-Ala2,N-methyl-Phe4,Glyol5]enkephalin (DAMGO) inhibits adenylate cyclase and stimulates p42/p44 mitogen-activated protein kinase (MAPK) phosphorylation. These results provide the evidence that ORL1/μR heterodimerization and the resulting impairment of μ receptor-activated signaling pathways may contribute to ORL1-mediated antiopioid effects in the brain.
原文英語
頁(從 - 到)1285-1294
頁數10
期刊Journal of Neurochemistry
92
發行號6
DOIs
出版狀態已發佈 - 3月 2005
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 細胞與分子神經科學

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