Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor

Yi Cheng Ho, Fwu Long Mi, Hsing W. Sung, Pi L. Kuo

研究成果: 雜誌貢獻文章

114 引文 (Scopus)

摘要

Controlled long-term release of basic fibroblast growth factor (bFGF) has shown a combined effect on the stimulation of regenerating a number of tissues including cartilage, nerve, skin and liver. In this study, three-dimensional scaffolds prepared from the polyelectrolyte complexes (PEC) of chitosan and alginate were developed for the delivery of bFGF. The bFGF-binding efficiency of the chitosan-alginate PEC scaffold, after being conjugated with high concentration of heparin (83.6 μg/mg scaffold), was increased up to 15 times higher than that of original scaffold (65.6 ng bFGF/mg scaffold vs. 4.5 ng bFGF/mg scaffold). The release of bFGF from the original scaffold was quick and the initial burst release was obvious. By functionalizing the scaffold with various concentrations of heparin (17.6 μg, 50.3 μg and 83.6 μg heparin/mg scaffold), the rate of bFGF release from the scaffold decreased in a controlled manner with reduced burst effect. The released bFGF retained its biological activity as assessed by the in vitro proliferation of human foreskin fibroblast (HFF). This study shows that a novel bFGF delivery system using the heparin-functionalized chitosan-alginate PEC scaffold exhibits controllable, long-term release of bFGF and could prevent the growth factor from inactivation.

原文英語
頁(從 - 到)69-75
頁數7
期刊International Journal of Pharmaceutics
376
發行號1-2
DOIs
出版狀態已發佈 - 七月 6 2009
對外發佈Yes

指紋

Chitosan
Fibroblast Growth Factor 2
Heparin
Intercellular Signaling Peptides and Proteins
alginic acid
Foreskin
Cartilage
Fibroblasts
Skin
Liver

ASJC Scopus subject areas

  • Pharmaceutical Science

引用此文

Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor. / Ho, Yi Cheng; Mi, Fwu Long; Sung, Hsing W.; Kuo, Pi L.

於: International Journal of Pharmaceutics, 卷 376, 編號 1-2, 06.07.2009, p. 69-75.

研究成果: 雜誌貢獻文章

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