Hemin Induced Heme Oxygenase-1 Over Expression Involves Nuclear Factor-E2 Related Factor-2, Nuclear Factor-κB and Extracellular Regulated Kinase: An Experimental Study in a Testicular Torsion-Detorsion Rodent Model

Stone Yang, Hung Jen Shih, Yung Chiong Chow, Pei-Shan Tsai, Chun Jen Huang

研究成果: 雜誌貢獻文章

11 引文 (Scopus)

摘要

Purpose: Nrf2 (nuclear factor-E2 related factor-2), nuclear factor-κB and mitogen-activated protein kinase, including extracellular regulated kinase, c-jun N-terminal kinase and p38 mitogen-activated protein kinase, are crucial for signal transduction. We previously reported that heme oxygenase-1 over expression induced by the heme oxygenase-1 inducer hemin protected testes from torsion-detorsion injury. In this study we elucidated which of these enzymes is involved in hemin induced heme oxygenase-1 over expression in testicular tissues after torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats (National Science Council, Taipei, Taiwan, Republic of China) were allocated to undergo testicular torsion-detorsion immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, a heme oxygenase-1 inhibitor. Another set of rats that underwent sham operation were used as controls. Testes were harvested 0 and 30 minutes after detorsion and enzyme expression was analyzed. Results: Hemin alone caused no significant effects on the expression of Nrf2, nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase. Similarly testicular torsion-detorsion injury caused no significant effects on Nrf2 expression. In the presence of torsion-detorsion injury hemin significantly up-regulated Nrf2 expression. Moreover, this effect was not affected by tin protoporphyrin. Unlike Nrf2, the expression of nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase was significantly up-regulated by testicular torsion-detorsion. Hemin significantly attenuated the testicular torsion-detorsion induced up-regulation of nuclear factor-κB and extracellular regulated kinase but not c-jun N-terminal kinase. The effects of hemin on nuclear factor-κB and extracellular regulated kinase were significantly reversed by tin protoporphyrin. However, the expression of p38 mitogen-activated protein kinase in rodent testes was not affected by these interventions. Conclusions: Hemin induced heme oxygenase-1 over expression in rodent testes after torsion-detorsion injury involves Nrf2, nuclear factor-κB and extracellular regulated kinase.
原文英語
頁(從 - 到)2456-2463
頁數8
期刊Journal of Urology
179
發行號6
DOIs
出版狀態已發佈 - 六月 2008

指紋

NF-E2-Related Factor 2
Spermatic Cord Torsion
Hemin
Heme Oxygenase-1
Rodentia
Phosphotransferases
JNK Mitogen-Activated Protein Kinases
Testis
Wounds and Injuries
p38 Mitogen-Activated Protein Kinases
Taiwan
Enzymes
Mitogen-Activated Protein Kinases
Sprague Dawley Rats
Signal Transduction
Up-Regulation

ASJC Scopus subject areas

  • Urology

引用此文

@article{29c7eb72d4aa42f3964782b6df9e20c5,
title = "Hemin Induced Heme Oxygenase-1 Over Expression Involves Nuclear Factor-E2 Related Factor-2, Nuclear Factor-κB and Extracellular Regulated Kinase: An Experimental Study in a Testicular Torsion-Detorsion Rodent Model",
abstract = "Purpose: Nrf2 (nuclear factor-E2 related factor-2), nuclear factor-κB and mitogen-activated protein kinase, including extracellular regulated kinase, c-jun N-terminal kinase and p38 mitogen-activated protein kinase, are crucial for signal transduction. We previously reported that heme oxygenase-1 over expression induced by the heme oxygenase-1 inducer hemin protected testes from torsion-detorsion injury. In this study we elucidated which of these enzymes is involved in hemin induced heme oxygenase-1 over expression in testicular tissues after torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats (National Science Council, Taipei, Taiwan, Republic of China) were allocated to undergo testicular torsion-detorsion immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, a heme oxygenase-1 inhibitor. Another set of rats that underwent sham operation were used as controls. Testes were harvested 0 and 30 minutes after detorsion and enzyme expression was analyzed. Results: Hemin alone caused no significant effects on the expression of Nrf2, nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase. Similarly testicular torsion-detorsion injury caused no significant effects on Nrf2 expression. In the presence of torsion-detorsion injury hemin significantly up-regulated Nrf2 expression. Moreover, this effect was not affected by tin protoporphyrin. Unlike Nrf2, the expression of nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase was significantly up-regulated by testicular torsion-detorsion. Hemin significantly attenuated the testicular torsion-detorsion induced up-regulation of nuclear factor-κB and extracellular regulated kinase but not c-jun N-terminal kinase. The effects of hemin on nuclear factor-κB and extracellular regulated kinase were significantly reversed by tin protoporphyrin. However, the expression of p38 mitogen-activated protein kinase in rodent testes was not affected by these interventions. Conclusions: Hemin induced heme oxygenase-1 over expression in rodent testes after torsion-detorsion injury involves Nrf2, nuclear factor-κB and extracellular regulated kinase.",
keywords = "heme oxygenase-1, rats, spermatic cord, spermatic cord torsion, Sprague-Dawley, testis",
author = "Stone Yang and Shih, {Hung Jen} and Chow, {Yung Chiong} and Pei-Shan Tsai and Huang, {Chun Jen}",
year = "2008",
month = "6",
doi = "10.1016/j.juro.2008.01.085",
language = "English",
volume = "179",
pages = "2456--2463",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Hemin Induced Heme Oxygenase-1 Over Expression Involves Nuclear Factor-E2 Related Factor-2, Nuclear Factor-κB and Extracellular Regulated Kinase

T2 - An Experimental Study in a Testicular Torsion-Detorsion Rodent Model

AU - Yang, Stone

AU - Shih, Hung Jen

AU - Chow, Yung Chiong

AU - Tsai, Pei-Shan

AU - Huang, Chun Jen

PY - 2008/6

Y1 - 2008/6

N2 - Purpose: Nrf2 (nuclear factor-E2 related factor-2), nuclear factor-κB and mitogen-activated protein kinase, including extracellular regulated kinase, c-jun N-terminal kinase and p38 mitogen-activated protein kinase, are crucial for signal transduction. We previously reported that heme oxygenase-1 over expression induced by the heme oxygenase-1 inducer hemin protected testes from torsion-detorsion injury. In this study we elucidated which of these enzymes is involved in hemin induced heme oxygenase-1 over expression in testicular tissues after torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats (National Science Council, Taipei, Taiwan, Republic of China) were allocated to undergo testicular torsion-detorsion immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, a heme oxygenase-1 inhibitor. Another set of rats that underwent sham operation were used as controls. Testes were harvested 0 and 30 minutes after detorsion and enzyme expression was analyzed. Results: Hemin alone caused no significant effects on the expression of Nrf2, nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase. Similarly testicular torsion-detorsion injury caused no significant effects on Nrf2 expression. In the presence of torsion-detorsion injury hemin significantly up-regulated Nrf2 expression. Moreover, this effect was not affected by tin protoporphyrin. Unlike Nrf2, the expression of nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase was significantly up-regulated by testicular torsion-detorsion. Hemin significantly attenuated the testicular torsion-detorsion induced up-regulation of nuclear factor-κB and extracellular regulated kinase but not c-jun N-terminal kinase. The effects of hemin on nuclear factor-κB and extracellular regulated kinase were significantly reversed by tin protoporphyrin. However, the expression of p38 mitogen-activated protein kinase in rodent testes was not affected by these interventions. Conclusions: Hemin induced heme oxygenase-1 over expression in rodent testes after torsion-detorsion injury involves Nrf2, nuclear factor-κB and extracellular regulated kinase.

AB - Purpose: Nrf2 (nuclear factor-E2 related factor-2), nuclear factor-κB and mitogen-activated protein kinase, including extracellular regulated kinase, c-jun N-terminal kinase and p38 mitogen-activated protein kinase, are crucial for signal transduction. We previously reported that heme oxygenase-1 over expression induced by the heme oxygenase-1 inducer hemin protected testes from torsion-detorsion injury. In this study we elucidated which of these enzymes is involved in hemin induced heme oxygenase-1 over expression in testicular tissues after torsion-detorsion injury. Materials and Methods: Adult male Sprague-Dawley rats (National Science Council, Taipei, Taiwan, Republic of China) were allocated to undergo testicular torsion-detorsion immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, a heme oxygenase-1 inhibitor. Another set of rats that underwent sham operation were used as controls. Testes were harvested 0 and 30 minutes after detorsion and enzyme expression was analyzed. Results: Hemin alone caused no significant effects on the expression of Nrf2, nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase. Similarly testicular torsion-detorsion injury caused no significant effects on Nrf2 expression. In the presence of torsion-detorsion injury hemin significantly up-regulated Nrf2 expression. Moreover, this effect was not affected by tin protoporphyrin. Unlike Nrf2, the expression of nuclear factor-κB, extracellular regulated kinase and c-jun N-terminal kinase was significantly up-regulated by testicular torsion-detorsion. Hemin significantly attenuated the testicular torsion-detorsion induced up-regulation of nuclear factor-κB and extracellular regulated kinase but not c-jun N-terminal kinase. The effects of hemin on nuclear factor-κB and extracellular regulated kinase were significantly reversed by tin protoporphyrin. However, the expression of p38 mitogen-activated protein kinase in rodent testes was not affected by these interventions. Conclusions: Hemin induced heme oxygenase-1 over expression in rodent testes after torsion-detorsion injury involves Nrf2, nuclear factor-κB and extracellular regulated kinase.

KW - heme oxygenase-1

KW - rats

KW - spermatic cord

KW - spermatic cord torsion

KW - Sprague-Dawley

KW - testis

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