Helicobacter pylori activates HMGB1 expression and recruits rage into lipid rafts to promote inflammation in gastric epithelial cells

Hwai Jeng Lin, Fang Yu Hsu, Wei Wei Chen, Che Hsin Lee, Ying Ju Lin, Yi Ywan M Chen, Chih Jung Chen, Mei Zi Huang, Min Chuan Kao, Yu An Chen, Hsin Chih Lai, Chih Ho Lai

研究成果: 雜誌貢獻文章同行評審

16 引文 斯高帕斯(Scopus)

摘要

Helicobacter pylori infection is associated with several gastrointestinal disorders in the human population worldwide. High-mobility group box 1 (HMGB1), a ubiquitous nuclear protein, mediates various inflammation functions. The interaction between HMGB1 and receptor for advanced glycation end-products (RAGE) triggers nuclear factor (NF)-κB expression, which in turn stimulates the release of proinflammatory cytokines, such as interleukin (IL)-8, and enhances the inflammatory response. However, how H. pylori activates HMGB1 expression and mobilizes RAGE into cholesterol-rich microdomains in gastric epithelial cells to promote inflammation has not been explored. In this study, we found that HMGB1 and RAGE expression increased significantly in H. pylori-infected cells compared with -uninfected cells. Blocking HMGB1 by neutralizing antibody abrogated H. pylori-elicited RAGE, suggesting that RAGE expression follows HMGB1 production, and silenced RAGE-attenuated H. pylori-mediated NF-κB activation and IL-8 production. Furthermore, significantly more RAGE was present in detergent-resistant membranes extracted from H. pylori-infected cells than in those from -uninfected cells, indicating that H. pylori exploited cholesterol to induce the HMGB1 signaling pathway. These results indicate that HMGB1 plays a crucial role in H. pylori-induced inflammation in gastric epithelial cells, which may be valuable in developing treatments for H. pylori-associated diseases.

原文英語
文章編號341
期刊Frontiers in Immunology
7
發行號SEP
DOIs
出版狀態已發佈 - 九月 9 2016

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學

指紋

深入研究「Helicobacter pylori activates HMGB1 expression and recruits rage into lipid rafts to promote inflammation in gastric epithelial cells」主題。共同形成了獨特的指紋。

引用此