Heatstroke effect on brain heme oxygenase-1 in rats

Ya Ting Wen, Tsung Ta Liu, Yuh Feng Lin, Chun Chi Chen, Woon Man Kung, Chi Chang Huang, Tien Jen Lin, Yuan Hung Wang, Li Wei

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Exposure to high environmental temperature leading to increased core body temperature above 40°C and central nervous system abnormalities such as convulsions, delirium, or coma is defined as heat stroke. Studies in humans and animals indicate that the heat shock responses of the host contribute to multiple organ injury and death during heat stroke. Heme oxygenase-1 (HO-1)—a stress-responsive enzyme that catabolizes heme into iron, carbon monoxide, and biliverdin—has an important role in the neuroprotective mechanism against ischemic stroke. Here, we investigated the role of endogenous HO-1 in heat-induced brain damage in rats. RT-PCR results revealed that levels of HO-1 mRNA peaked at 0 h after heat exposure and immunoblot analysis revealed that the maximal protein expression occurred at 1 h post-heat exposure. Subsequently, we detected the HO-1 expression in the cortical brain cells and revealed the neuronal cell morphology. In conclusion, HO-1 is a potent protective molecule against heat-induced brain damage. Manipulation of HO-1 may provide a potential therapeutic approach for heat-related diseases.
原文英語
頁(從 - 到)737-741
頁數5
期刊International Journal of Medical Sciences
12
發行號9
DOIs
出版狀態已發佈 - 2015

指紋

Heat Stroke
Heme Oxygenase-1
Hot Temperature
Brain
Nervous System Malformations
Heat-Shock Response
Delirium
Multiple Trauma
Carbon Monoxide
Coma
Body Temperature
Heme
Seizures
Iron
Central Nervous System
Stroke
Polymerase Chain Reaction
Messenger RNA
Temperature
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Heatstroke effect on brain heme oxygenase-1 in rats. / Wen, Ya Ting; Liu, Tsung Ta; Lin, Yuh Feng; Chen, Chun Chi; Kung, Woon Man; Huang, Chi Chang; Lin, Tien Jen; Wang, Yuan Hung; Wei, Li.

於: International Journal of Medical Sciences, 卷 12, 編號 9, 2015, p. 737-741.

研究成果: 雜誌貢獻文章

Wen, Ya Ting ; Liu, Tsung Ta ; Lin, Yuh Feng ; Chen, Chun Chi ; Kung, Woon Man ; Huang, Chi Chang ; Lin, Tien Jen ; Wang, Yuan Hung ; Wei, Li. / Heatstroke effect on brain heme oxygenase-1 in rats. 於: International Journal of Medical Sciences. 2015 ; 卷 12, 編號 9. 頁 737-741.
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AU - Lin, Yuh Feng

AU - Chen, Chun Chi

AU - Kung, Woon Man

AU - Huang, Chi Chang

AU - Lin, Tien Jen

AU - Wang, Yuan Hung

AU - Wei, Li

PY - 2015

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N2 - Exposure to high environmental temperature leading to increased core body temperature above 40°C and central nervous system abnormalities such as convulsions, delirium, or coma is defined as heat stroke. Studies in humans and animals indicate that the heat shock responses of the host contribute to multiple organ injury and death during heat stroke. Heme oxygenase-1 (HO-1)—a stress-responsive enzyme that catabolizes heme into iron, carbon monoxide, and biliverdin—has an important role in the neuroprotective mechanism against ischemic stroke. Here, we investigated the role of endogenous HO-1 in heat-induced brain damage in rats. RT-PCR results revealed that levels of HO-1 mRNA peaked at 0 h after heat exposure and immunoblot analysis revealed that the maximal protein expression occurred at 1 h post-heat exposure. Subsequently, we detected the HO-1 expression in the cortical brain cells and revealed the neuronal cell morphology. In conclusion, HO-1 is a potent protective molecule against heat-induced brain damage. Manipulation of HO-1 may provide a potential therapeutic approach for heat-related diseases.

AB - Exposure to high environmental temperature leading to increased core body temperature above 40°C and central nervous system abnormalities such as convulsions, delirium, or coma is defined as heat stroke. Studies in humans and animals indicate that the heat shock responses of the host contribute to multiple organ injury and death during heat stroke. Heme oxygenase-1 (HO-1)—a stress-responsive enzyme that catabolizes heme into iron, carbon monoxide, and biliverdin—has an important role in the neuroprotective mechanism against ischemic stroke. Here, we investigated the role of endogenous HO-1 in heat-induced brain damage in rats. RT-PCR results revealed that levels of HO-1 mRNA peaked at 0 h after heat exposure and immunoblot analysis revealed that the maximal protein expression occurred at 1 h post-heat exposure. Subsequently, we detected the HO-1 expression in the cortical brain cells and revealed the neuronal cell morphology. In conclusion, HO-1 is a potent protective molecule against heat-induced brain damage. Manipulation of HO-1 may provide a potential therapeutic approach for heat-related diseases.

KW - Cortical brain

KW - Heat stroke

KW - Heme oxygenase-1

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