Heat shock protein70 is implicated in modulating NF-κ B activation in alveolar macrophages of patients with active pulmonary tuberculosis

Chun Hua Wang, Pai Chien Chou, Fu Tsai Chung, Horng Chyuan Lin, Kuo Hsiung Huang, Han Pin Kuo

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)

摘要

Heat shock proteins (HSPs) have been shown to modulate NF-κ B activation. It is unknown whether HSP70 plays a role in modulating NF-κ B-mediated pro-inflammatory cytokines released from alveolar macrophage (AM) of patients with active pulmonary tuberculosis (TB). Peripheral blood monocytes (PBMs) and AM were sampled from nineteen active TB patients and 14 healthy individuals. HSP70 expression was 3-fold higher in AMs of active TB patients than normal subjects, and declined after receiving 3-month anti-TB treatment. Overexpression of HSP70 by transfection with HSP70 plasmid decreased p-Iκ Bα and p65 NF-κ B activities. Inhibition of NF-κ B activation using NF-κ B or MAPK inhibitors increased HSP70 expression in AM of TB patients. Blocking p38- or ERK-MAPK decreased NF-κ B and Iκ B activities, leading to up-regulated HSP70 expression. Overexpression of HSP70 alone or with p38 or ERK inhibitors decreased TNF-α (57%, 83% and 74%, respectively) and IL-6 (53%, 70%, and 67%, respectively) release from macrophages of TB patients. In conclusion, HSP70 modulates NF-κ B activation in AM of TB patients, through inhibiting Iκ B-α phosphorylation or acting as a chaperon molecule to prevent NF-κ B binding to the target genes by facilitating degradation. The upregulated HSP70 may suppress the release of pro-inflammatory cytokines during active PTB infection, and prevent overwhelming tissue damage.
原文英語
文章編號1214
期刊Scientific Reports
7
發行號1
DOIs
出版狀態已發佈 - 十二月 1 2017

ASJC Scopus subject areas

  • General

指紋 深入研究「Heat shock protein70 is implicated in modulating NF-κ B activation in alveolar macrophages of patients with active pulmonary tuberculosis」主題。共同形成了獨特的指紋。

引用此