Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Tsai Mu Cheng, Simon J T Mao, Shiau Ting Lai, Chia Ching Chang, Ming Chi Yang, Nai Chi Chen, Shiu Ching Chou, Ju Pin Pan

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)

摘要

Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.
原文英語
頁(從 - 到)303-316
頁數14
期刊Free Radical Research
45
發行號3
DOIs
出版狀態已發佈 - 三月 2011
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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