H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM

Min Wei Chen, Kuo Tai Hua, Hsin Jung Kao, Chia Chun Chi, Lin Hung Wei, Gunnar Johansson, Shine Gwo Shiah, Pai Sheng Chen, Yung Ming Jeng, Tsu Yao Cheng, Tsung Ching Lai, Jeng Shou Chang, Yi Hua Jan, Ming Hsien Chien, Chih Jen Yang, Ming Shyan Huang, Michael Hsiao, Min Liang Kuo

研究成果: 雜誌貢獻文章

214 引文 斯高帕斯(Scopus)

摘要

G9a is a mammalian histone methyltransferase that contributes to the epigenetic silencing of tumor suppressor genes. Emerging evidence suggests that G9a is required to maintain the malignant phenotype, but the role of G9a function in mediating tumor metastasis has not been explored. Here, we show that G9a is expressed in aggressive lung cancer cells, and its elevated expression correlates with poor prognosis. RNAi-mediated knockdown of G9a in highly invasive lung cancer cells inhibited cell migration and invasion in vitro and metastasis in vivo. Conversely, ectopic G9a expression in weakly invasive lung cancer cells increased motility and metastasis. Mechanistic investigations suggested that repression of the cell adhesion molecule Ep-CAM mediated the effects of G9a. First, RNAi-mediated knockdown of Ep-CAM partially relieved metastasis suppression imposed by G9a suppression. Second, an inverse correlation between G9a and Ep-CAM expression existed in primary lung cancer. Third, Ep-CAM repression was associated with promoter methylation and an enrichment for dimethylated histone H3K9. G9a knockdown reduced the levels of H3K9 dimethylation and decreased the recruitment of the transcriptional cofactors HP1, DNMT1, and HDAC1 to the Ep-CAM promoter. Our findings establish a functional contribution of G9a overexpression with concomitant dysregulation of epigenetic pathways in lung cancer progression.

原文英語
頁(從 - 到)7830-7840
頁數11
期刊Cancer Research
70
發行號20
DOIs
出版狀態已發佈 - 十月 15 2010

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Chen, M. W., Hua, K. T., Kao, H. J., Chi, C. C., Wei, L. H., Johansson, G., Shiah, S. G., Chen, P. S., Jeng, Y. M., Cheng, T. Y., Lai, T. C., Chang, J. S., Jan, Y. H., Chien, M. H., Yang, C. J., Huang, M. S., Hsiao, M., & Kuo, M. L. (2010). H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM. Cancer Research, 70(20), 7830-7840. https://doi.org/10.1158/0008-5472.CAN-10-0833