G.T wobble base-pairing in Z-DNA at 1.0 A atomic resolution: the crystal structure of d(CGCGTG).

P. S. Ho, C. A. Frederick, G. J. Quigley, G. A. van der Marel, J. H. van Boom, A. H. Wang, A. Rich

研究成果: 雜誌貢獻文章同行評審

80 引文 斯高帕斯(Scopus)

摘要

The DNA oligomer d(CGCGTG) crystallizes as a Z-DNA double helix containing two guanine-thymine base pair mismatches of the wobble type. The crystal diffracts to 1 A resolution and the structure has been solved and refined. At this resolution, a large amount of information is revealed about the organization of the water molecules in the lattice generally and more specifically around the wobble base pairs. By comparing this structure with the analogous high resolution structure of d(CGCGCG) we can visualize the structural changes as well as the reorganization of the solvent molecules associated with wobble base pairing. There is only a small distortion of the Z-DNA backbone resulting from introduction of the GT mismatched base pairs. The water molecules cluster around the wobble base pair taking up all of the hydrogen bonding capabilities of the bases due to wobble pairing. These bridging water molecules serve to stabilize the base-base interaction and, thus, may be generally important for base mispairing either in DNA or in RNA molecules.

原文英語
頁(從 - 到)3617-3623
頁數7
期刊EMBO Journal
4
發行號13 A
DOIs
出版狀態已發佈 - 12月 16 1985
對外發佈

ASJC Scopus subject areas

  • 神經科學 (全部)
  • 分子生物學
  • 生物化學、遺傳與分子生物學 (全部)
  • 免疫學與微生物學 (全部)

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