Grape seed proanthocyanidin extract chelates iron and attenuates the toxic effects of 6-hydroxydopamine: Implications for Parkinson's disease

Tzu H. Wu, Jiahn Haur Liao, Feng-Lin Hsu, Huey R. Wu, Chuan K. Shen, J. M P Yuann, Shui Tein Chen

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

Proanthocyanidins are potent antioxidants associated with protection against diseases. We tested the reducing capacity, iron chelating activity, and anti-auto-oxidation ability of grape seed proanthocyanidin extract (GSPE). The mechanisms underlying GSPE attenuation of oxidative processes induced by 6-hydroxydopamine (6-OHDA), a neurotoxin used to induce Parkinson's disease, were investigated in cell-based systems. At high concentrations, GSPE (50 μg/μL) was a mild pro-oxidant in a Fenton-type reaction. GSPE (300 μg/mL) was as potent as 30 μM deferoxamine in its iron-chelating capacity, and as efficient as 5 mM ascorbic acid in delaying 6-OHDA auto-oxidation. In PC-12 cell cultures, 100 and 300 μg/mL GSPE significantly protected (P <0.05) cells from 6-OHDA-induced (400 μM) toxicity. GSPE-induced cytoprotection is enhanced by a nitric oxide synthase inhibitor (NOSI), implying that the cytoprotective effect of GSPE does not require NOS activation. In conclusion, the iron-chelating activity of GSPE minimizes its pro-oxidant activity and delays 6-OHDA auto-oxidation to provide cytoprotection.

原文英語
頁(從 - 到)244-262
頁數19
期刊Journal of Food Biochemistry
34
發行號2
DOIs
出版狀態已發佈 - 四月 2010

指紋

Grape Seed Extract
Iron Chelating Agents
grape seeds
Parkinson disease
Poisons
Oxidopamine
chelates
proanthocyanidins
Parkinson Disease
iron
extracts
automobiles
Cytoprotection
Iron
oxidation
oxidants
Reactive Oxygen Species
deferoxamine
Proanthocyanidins
Deferoxamine

ASJC Scopus subject areas

  • Food Science
  • Biophysics
  • Cell Biology
  • Pharmacology

引用此文

Grape seed proanthocyanidin extract chelates iron and attenuates the toxic effects of 6-hydroxydopamine : Implications for Parkinson's disease. / Wu, Tzu H.; Liao, Jiahn Haur; Hsu, Feng-Lin; Wu, Huey R.; Shen, Chuan K.; Yuann, J. M P; Chen, Shui Tein.

於: Journal of Food Biochemistry, 卷 34, 編號 2, 04.2010, p. 244-262.

研究成果: 雜誌貢獻文章

Wu, Tzu H. ; Liao, Jiahn Haur ; Hsu, Feng-Lin ; Wu, Huey R. ; Shen, Chuan K. ; Yuann, J. M P ; Chen, Shui Tein. / Grape seed proanthocyanidin extract chelates iron and attenuates the toxic effects of 6-hydroxydopamine : Implications for Parkinson's disease. 於: Journal of Food Biochemistry. 2010 ; 卷 34, 編號 2. 頁 244-262.
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