Glutamine attenuates hyperoxia-induced acute lung injury in mice

Wann Cherng Perng, Kun Lun Huang, Min Hui Li, Ching Wang Hsu, Shih Hung Tsai, Shi Jye Chu, Deh Ming Chang

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

1. Glutamine is an amino acid that is used to treat various diseases. Glutamine has been reported to have protective effects in human pulmonary epithelia-like cells exposed to hyperoxia. However, the effects of glutamine in hyperoxia-induced lung injury have not been investigated in vivo. 2. Mice treated with saline or glutamine [(750 mg/kg) intravenously] were randomly exposed to hyperoxia for 48 or 72 h. Control mice treated with saline or glutamine were exposed to room air. Cytokine levels in bronchoalveolar lavage fluid (BALF), heat shock protein (HSP) 70, the wet/dry (W/D) weight ratio, malondialdehyde (MDA) levels, myeloperoxidase (MPO) activity and pathoglogical findings in lung tissue were evaluated to determine the effects of glutamine on acute lung injury. In addition, survival was monitored. 3. Lung expression of HSP70 was significantly enhanced in both the control (room air) and 48 and 72 h hyperoxic glutamine-treated mice. The W/D ratio, BALF concentrations of tumour necrosis factor-α and interleukin-6, MDA levels, MPO activity, neutrophil infiltration and interstitial oedema in lung tissue were significantly lower at 48 and 72 h of hyperoxia in glutamine-treated mice compared with saline-treated mice. 4. In a separate series of experiments evaluating survival, after 96 h continuous exposure to hyperoxia, all saline-treated mice died. In contrast, all glutamine-treated mice died after 108 h exposure to hyperoxia. 5. The data suggest that glutamine administered to mice during hyperoxia has a protective effect against hyperoxia-induced acute lung injury and improves survival.

原文英語
頁(從 - 到)56-61
頁數6
期刊Clinical and Experimental Pharmacology and Physiology
37
發行號1
DOIs
出版狀態已發佈 - 一月 2010
對外發佈Yes

指紋

Hyperoxia
Acute Lung Injury
Glutamine
Lung
Bronchoalveolar Lavage Fluid
Malondialdehyde
Peroxidase
Air
HSP70 Heat-Shock Proteins
Survival
Neutrophil Infiltration
Lung Injury
Interleukin-6
Edema
Epithelium
Tumor Necrosis Factor-alpha
Cytokines
Amino Acids
Weights and Measures

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Pharmacology

引用此文

Glutamine attenuates hyperoxia-induced acute lung injury in mice. / Perng, Wann Cherng; Huang, Kun Lun; Li, Min Hui; Hsu, Ching Wang; Tsai, Shih Hung; Chu, Shi Jye; Chang, Deh Ming.

於: Clinical and Experimental Pharmacology and Physiology, 卷 37, 編號 1, 01.2010, p. 56-61.

研究成果: 雜誌貢獻文章

Perng, Wann Cherng ; Huang, Kun Lun ; Li, Min Hui ; Hsu, Ching Wang ; Tsai, Shih Hung ; Chu, Shi Jye ; Chang, Deh Ming. / Glutamine attenuates hyperoxia-induced acute lung injury in mice. 於: Clinical and Experimental Pharmacology and Physiology. 2010 ; 卷 37, 編號 1. 頁 56-61.
@article{5ed30706da994edb83f922bcceac931b,
title = "Glutamine attenuates hyperoxia-induced acute lung injury in mice",
abstract = "1. Glutamine is an amino acid that is used to treat various diseases. Glutamine has been reported to have protective effects in human pulmonary epithelia-like cells exposed to hyperoxia. However, the effects of glutamine in hyperoxia-induced lung injury have not been investigated in vivo. 2. Mice treated with saline or glutamine [(750 mg/kg) intravenously] were randomly exposed to hyperoxia for 48 or 72 h. Control mice treated with saline or glutamine were exposed to room air. Cytokine levels in bronchoalveolar lavage fluid (BALF), heat shock protein (HSP) 70, the wet/dry (W/D) weight ratio, malondialdehyde (MDA) levels, myeloperoxidase (MPO) activity and pathoglogical findings in lung tissue were evaluated to determine the effects of glutamine on acute lung injury. In addition, survival was monitored. 3. Lung expression of HSP70 was significantly enhanced in both the control (room air) and 48 and 72 h hyperoxic glutamine-treated mice. The W/D ratio, BALF concentrations of tumour necrosis factor-α and interleukin-6, MDA levels, MPO activity, neutrophil infiltration and interstitial oedema in lung tissue were significantly lower at 48 and 72 h of hyperoxia in glutamine-treated mice compared with saline-treated mice. 4. In a separate series of experiments evaluating survival, after 96 h continuous exposure to hyperoxia, all saline-treated mice died. In contrast, all glutamine-treated mice died after 108 h exposure to hyperoxia. 5. The data suggest that glutamine administered to mice during hyperoxia has a protective effect against hyperoxia-induced acute lung injury and improves survival.",
keywords = "Acute lung injury, Glutamine, Hyperoxia",
author = "Perng, {Wann Cherng} and Huang, {Kun Lun} and Li, {Min Hui} and Hsu, {Ching Wang} and Tsai, {Shih Hung} and Chu, {Shi Jye} and Chang, {Deh Ming}",
year = "2010",
month = "1",
doi = "10.1111/j.1440-1681.2009.05239.x",
language = "English",
volume = "37",
pages = "56--61",
journal = "Clinical and Experimental Pharmacology and Physiology",
issn = "0305-1870",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Glutamine attenuates hyperoxia-induced acute lung injury in mice

AU - Perng, Wann Cherng

AU - Huang, Kun Lun

AU - Li, Min Hui

AU - Hsu, Ching Wang

AU - Tsai, Shih Hung

AU - Chu, Shi Jye

AU - Chang, Deh Ming

PY - 2010/1

Y1 - 2010/1

N2 - 1. Glutamine is an amino acid that is used to treat various diseases. Glutamine has been reported to have protective effects in human pulmonary epithelia-like cells exposed to hyperoxia. However, the effects of glutamine in hyperoxia-induced lung injury have not been investigated in vivo. 2. Mice treated with saline or glutamine [(750 mg/kg) intravenously] were randomly exposed to hyperoxia for 48 or 72 h. Control mice treated with saline or glutamine were exposed to room air. Cytokine levels in bronchoalveolar lavage fluid (BALF), heat shock protein (HSP) 70, the wet/dry (W/D) weight ratio, malondialdehyde (MDA) levels, myeloperoxidase (MPO) activity and pathoglogical findings in lung tissue were evaluated to determine the effects of glutamine on acute lung injury. In addition, survival was monitored. 3. Lung expression of HSP70 was significantly enhanced in both the control (room air) and 48 and 72 h hyperoxic glutamine-treated mice. The W/D ratio, BALF concentrations of tumour necrosis factor-α and interleukin-6, MDA levels, MPO activity, neutrophil infiltration and interstitial oedema in lung tissue were significantly lower at 48 and 72 h of hyperoxia in glutamine-treated mice compared with saline-treated mice. 4. In a separate series of experiments evaluating survival, after 96 h continuous exposure to hyperoxia, all saline-treated mice died. In contrast, all glutamine-treated mice died after 108 h exposure to hyperoxia. 5. The data suggest that glutamine administered to mice during hyperoxia has a protective effect against hyperoxia-induced acute lung injury and improves survival.

AB - 1. Glutamine is an amino acid that is used to treat various diseases. Glutamine has been reported to have protective effects in human pulmonary epithelia-like cells exposed to hyperoxia. However, the effects of glutamine in hyperoxia-induced lung injury have not been investigated in vivo. 2. Mice treated with saline or glutamine [(750 mg/kg) intravenously] were randomly exposed to hyperoxia for 48 or 72 h. Control mice treated with saline or glutamine were exposed to room air. Cytokine levels in bronchoalveolar lavage fluid (BALF), heat shock protein (HSP) 70, the wet/dry (W/D) weight ratio, malondialdehyde (MDA) levels, myeloperoxidase (MPO) activity and pathoglogical findings in lung tissue were evaluated to determine the effects of glutamine on acute lung injury. In addition, survival was monitored. 3. Lung expression of HSP70 was significantly enhanced in both the control (room air) and 48 and 72 h hyperoxic glutamine-treated mice. The W/D ratio, BALF concentrations of tumour necrosis factor-α and interleukin-6, MDA levels, MPO activity, neutrophil infiltration and interstitial oedema in lung tissue were significantly lower at 48 and 72 h of hyperoxia in glutamine-treated mice compared with saline-treated mice. 4. In a separate series of experiments evaluating survival, after 96 h continuous exposure to hyperoxia, all saline-treated mice died. In contrast, all glutamine-treated mice died after 108 h exposure to hyperoxia. 5. The data suggest that glutamine administered to mice during hyperoxia has a protective effect against hyperoxia-induced acute lung injury and improves survival.

KW - Acute lung injury

KW - Glutamine

KW - Hyperoxia

UR - http://www.scopus.com/inward/record.url?scp=74049126635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74049126635&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1681.2009.05239.x

DO - 10.1111/j.1440-1681.2009.05239.x

M3 - Article

C2 - 19566832

AN - SCOPUS:74049126635

VL - 37

SP - 56

EP - 61

JO - Clinical and Experimental Pharmacology and Physiology

JF - Clinical and Experimental Pharmacology and Physiology

SN - 0305-1870

IS - 1

ER -