Glucose reduces the osmopressor response in connection with the tyrosine phosphorylation of focal adhesion kinase in red blood cells

You Hsiang Chu, You Hsiang Chu, Ying Hsuan Tai, Ying Hsuan Tai, Ying Hsuan Tai, Chun Chang Yeh, Mei Yung Tsou, Mei Yung Tsou, Herng Sheng Lee, Shung Tai Ho, Shung Tai Ho, Min Hui Li, Min Hui Li, Tso Chou Lin, Chih Cherng Lu, Chih Cherng Lu, Chih Cherng Lu

研究成果: 雜誌貢獻文章同行評審

摘要

Glucose ingestion attenuates the water ingestion-induced increase in the total peripheral vascular resistance and orthostatic tolerance. We investigated the gastrointestinal physiology of glucose by examining the effect of glucose ingestion on the functional expression of focal adhesion kinase (FAK) in red blood cell (RBC) membrane. This study was performed in 24 young, healthy subjects. Blood samples were collected at 5 min before and 25 min and 50 min after an ingestion of 10% glucose water 500 mL, water 500 mL, or normal saline 500 mL. We determined glucose and osmolality in plasma, and phosphorylation of aquaporin 1 (AQP1), glucose transporter 1 (Glut1), and FAK in RBC membrane. Our results showed that glucose ingestion reduced the rise of peripheral vascular resistance after water ingestion and upregulated the serine phosphorylation of Glut1. It also lowered both the serine phosphorylation of FAK and tyrosine phosphorylation of AQP1, compared with the ingestion of either water or saline. In an ex vivo experiment, glucose activated the Glut1 receptor and subsequently reduced the expression of FAK compared with 0.8% saline alone. We concluded that glucose activates Glut1 and subsequently lowers the functional expression of FAK, a cytoskeleton protein of RBCs. The functional change in the RBC membrane proteins in connection with the attenuation of osmopressor response may elucidate the pathophysiology of glucose in postprandial hypotension.
原文英語
頁(從 - 到)128-136
頁數9
期刊Chinese Journal of Physiology
63
發行號3
DOIs
出版狀態已發佈 - 5月 1 2020

ASJC Scopus subject areas

  • 生理學
  • 生理學(醫學)

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