Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes

Rajkumar Devasenathipathy, Veerappan Mani, Shen Ming Chen, Sheng Tung Huang, Tsung Tao Huang, Chun Mao Lin, Kuo Yuan Hwa, Ting Yo Chen, Bo Jun Chen

研究成果: 雜誌貢獻文章

67 引文 (Scopus)

摘要

Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.

原文英語
頁(從 - 到)40-45
頁數6
期刊Enzyme and Microbial Technology
78
DOIs
出版狀態已發佈 - 十月 1 2015

指紋

Glucose Oxidase
Carbon Nanotubes
Glucose oxidase
Graphite
Biosensing Techniques
Biosensors
Gold
Nanoparticles
Graphene
Glucose
Carbon nanotubes
Electrons
Biopolymers
Multiwalled carbon nanotubes (MWCN)
Sensors
Fourier Transform Infrared Spectroscopy
Motion Pictures
X-Ray Diffraction
Electron Scanning Microscopy
Oxidation-Reduction

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

引用此文

Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes. / Devasenathipathy, Rajkumar; Mani, Veerappan; Chen, Shen Ming; Huang, Sheng Tung; Huang, Tsung Tao; Lin, Chun Mao; Hwa, Kuo Yuan; Chen, Ting Yo; Chen, Bo Jun.

於: Enzyme and Microbial Technology, 卷 78, 01.10.2015, p. 40-45.

研究成果: 雜誌貢獻文章

Devasenathipathy, Rajkumar ; Mani, Veerappan ; Chen, Shen Ming ; Huang, Sheng Tung ; Huang, Tsung Tao ; Lin, Chun Mao ; Hwa, Kuo Yuan ; Chen, Ting Yo ; Chen, Bo Jun. / Glucose biosensor based on glucose oxidase immobilized at gold nanoparticles decorated graphene-carbon nanotubes. 於: Enzyme and Microbial Technology. 2015 ; 卷 78. 頁 40-45.
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abstract = "Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.",
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author = "Rajkumar Devasenathipathy and Veerappan Mani and Chen, {Shen Ming} and Huang, {Sheng Tung} and Huang, {Tsung Tao} and Lin, {Chun Mao} and Hwa, {Kuo Yuan} and Chen, {Ting Yo} and Chen, {Bo Jun}",
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AU - Mani, Veerappan

AU - Chen, Shen Ming

AU - Huang, Sheng Tung

AU - Huang, Tsung Tao

AU - Lin, Chun Mao

AU - Hwa, Kuo Yuan

AU - Chen, Ting Yo

AU - Chen, Bo Jun

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N2 - Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.

AB - Biopolymer pectin stabilized gold nanoparticles were prepared at graphene and multiwalled carbon nanotubes (GR-MWNTs/AuNPs) and employed for the determination of glucose. The formation of GR-MWNTs/AuNPs was confirmed by scanning electron microscopy, X-ray diffraction, UV-vis and FTIR spectroscopy methods. Glucose oxidase (GOx) was successfully immobilized on GR-MWNTs/AuNPs film and direct electron transfer of GOx was investigated. GOx exhibits highly enhanced redox peaks with formal potential of -0.40V (vs. Ag/AgCl). The amount of electroactive GOx and electron transfer rate constant were found to be 10.5×10-10molcm-2 and 3.36s-1, respectively, which were significantly larger than the previous reports. The fabricated amperometric glucose biosensor sensitively detects glucose and showed two linear ranges: (1) 10μM-2mM with LOD of 4.1μM, (2) 2mM-5.2mM with LOD of 0.95mM. The comparison of the biosensor performance with reported sensors reveals the significant improvement in overall sensor performance. Moreover, the biosensor exhibited appreciable stability, repeatability, reproducibility and practicality. The other advantages of the fabricated biosensor are simple and green fabrication approach, roughed and stable electrode surface, fast in sensing and highly reproducible.

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