Global analysis of differentially expressed genes in early gestational decidua and chorionic villi using a 9600 human cDNA microarray

Huei Wen Chen, Jeremy J W Chen, Chii Ruey Tzeng, Han Ni Li, Shu Ju Chang, Yu Fei Cheng, Ching-Wen Chang, Ruey Sheng Wang, Pan Chyr Yang, Yuan Teh Lee

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)

摘要

The global gene expression profiles of the decidua and chorionic villi of early human pregnancies were analysed by using cDNA microarray technology. Decidual and villous placental tissues were obtained from first trimester abortus and mRNA was extracted for cDNA microarray analysis. The human cDNA microarray [9600 clones, including known regulatory genes and expressed sequence tags (EST)] with colorimetric detection was used to identify differentially expressed genes between early gestational decidua and villi. According to cDNA microarray analysis, we have identified 641 genes with highly expressed mRNA in both decidua and villi, 49 genes with higher expressions in decidua, and 75 genes with higher expression in chorionic villi. These differentially expressed genes were further grouped into categories by their putative functions, including: cell growth-related factors, hormones/cytokines, cell adhesion molecules, signal transduction molecules, apoptosis-related factors, cytoskeleton/extracellular matrix proteins, and EST. Immunohistochemical stainings of cathepsin L, leukaemia inhibitory factor-receptor, and proliferative cell nuclear antigen showed results consistent with the microarray data. Identification of the differentially expressed genes between decidua and villi by microarray provide a global profiling of the gene expression pattern. This work adds to our understanding of placentation by reporting the gene expression profiles during first trimester human pregnancies using cDNA microarray.

原文英語
頁(從 - 到)475-484
頁數10
期刊Molecular Human Reproduction
8
發行號5
出版狀態已發佈 - 2002

ASJC Scopus subject areas

  • 婦產科
  • 遺傳學
  • 發展生物學
  • 胚胎學
  • 細胞生物學

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