Giant polypeptides of skeletal muscle titin: Sedimentation equilibrium in guanidine hydrochloride

Gary P. Kurzban, Kuan Wang

研究成果: 雜誌貢獻文章同行評審

60 引文 斯高帕斯(Scopus)

摘要

Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1 S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 × 106.

原文英語
頁(從 - 到)1155-1161
頁數7
期刊Biochemical and Biophysical Research Communications
150
發行號3
DOIs
出版狀態已發佈 - 二月 15 1988
對外發佈Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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