Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene

Shu Chuan Tsai, Jiann Horng Leu, Chih Ming Chou, Maw Sheng Yeh, Fore Lien Huang, Chang Jen Huang

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

The CDC37 gene was isolated from a round-spotted pufferfish genomic library and characterized. This gene is composed of nine exons spanning 3.5 kb. Exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. By 5'-RACE (rapid amplication of cDNA ends) and sequence analysis, we deduced the promoter region for the CDC37 gene and found that it does not contain typical TATA or CCAAT box. The 1.8 kb DNA fragment upstream of the putative transcription initiation site contains numerous potential binding sites for transcription factors including CREB, E2A, Ets-1, GATA, NF-IL6 and PEA3. When this DNA fragment was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF cell line, it could drive the synthesis of CAT enzyme four times more efficiently than the promoterless pCAT-Basic did. In addition, the CDC37 gene is linked to the TYK2 gene in a tail-to-head manner with a small intergenic region of 292 bp. Copyright (C) 2000 Elsevier Science B.V.
原文英語
頁(從 - 到)144-148
頁數5
期刊Biochimica et Biophysica Acta - Gene Structure and Expression
1494
發行號1-2
DOIs
出版狀態已發佈 - 十一月 15 2000
對外發佈Yes

指紋

Tetraodontiformes
Genetic Promoter Regions
Genes
Exons
Chloramphenicol O-Acetyltransferase
CCAAT-Enhancer-Binding Protein-beta
Intergenic DNA
Genomic Library
Carps
5' Untranslated Regions
Transcription Initiation Site
DNA
Reporter Genes
Sequence Analysis
Tail
Transcription Factors
Complementary DNA
Binding Sites
Cell Line
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

引用此文

Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene. / Tsai, Shu Chuan; Leu, Jiann Horng; Chou, Chih Ming; Yeh, Maw Sheng; Huang, Fore Lien; Huang, Chang Jen.

於: Biochimica et Biophysica Acta - Gene Structure and Expression, 卷 1494, 編號 1-2, 15.11.2000, p. 144-148.

研究成果: 雜誌貢獻文章

Tsai, Shu Chuan ; Leu, Jiann Horng ; Chou, Chih Ming ; Yeh, Maw Sheng ; Huang, Fore Lien ; Huang, Chang Jen. / Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene. 於: Biochimica et Biophysica Acta - Gene Structure and Expression. 2000 ; 卷 1494, 編號 1-2. 頁 144-148.
@article{c355a3fdefa14ef3bcc688565736feba,
title = "Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene",
abstract = "The CDC37 gene was isolated from a round-spotted pufferfish genomic library and characterized. This gene is composed of nine exons spanning 3.5 kb. Exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. By 5'-RACE (rapid amplication of cDNA ends) and sequence analysis, we deduced the promoter region for the CDC37 gene and found that it does not contain typical TATA or CCAAT box. The 1.8 kb DNA fragment upstream of the putative transcription initiation site contains numerous potential binding sites for transcription factors including CREB, E2A, Ets-1, GATA, NF-IL6 and PEA3. When this DNA fragment was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF cell line, it could drive the synthesis of CAT enzyme four times more efficiently than the promoterless pCAT-Basic did. In addition, the CDC37 gene is linked to the TYK2 gene in a tail-to-head manner with a small intergenic region of 292 bp. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "CDC37, Genomic structure, Promoter, Round-spotted pufferfish (Tetraodon fluviatilis)",
author = "Tsai, {Shu Chuan} and Leu, {Jiann Horng} and Chou, {Chih Ming} and Yeh, {Maw Sheng} and Huang, {Fore Lien} and Huang, {Chang Jen}",
year = "2000",
month = "11",
day = "15",
doi = "10.1016/S0167-4781(00)00138-X",
language = "English",
volume = "1494",
pages = "144--148",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "1-2",

}

TY - JOUR

T1 - Genomic organization and the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) CDC37 gene

AU - Tsai, Shu Chuan

AU - Leu, Jiann Horng

AU - Chou, Chih Ming

AU - Yeh, Maw Sheng

AU - Huang, Fore Lien

AU - Huang, Chang Jen

PY - 2000/11/15

Y1 - 2000/11/15

N2 - The CDC37 gene was isolated from a round-spotted pufferfish genomic library and characterized. This gene is composed of nine exons spanning 3.5 kb. Exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. By 5'-RACE (rapid amplication of cDNA ends) and sequence analysis, we deduced the promoter region for the CDC37 gene and found that it does not contain typical TATA or CCAAT box. The 1.8 kb DNA fragment upstream of the putative transcription initiation site contains numerous potential binding sites for transcription factors including CREB, E2A, Ets-1, GATA, NF-IL6 and PEA3. When this DNA fragment was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF cell line, it could drive the synthesis of CAT enzyme four times more efficiently than the promoterless pCAT-Basic did. In addition, the CDC37 gene is linked to the TYK2 gene in a tail-to-head manner with a small intergenic region of 292 bp. Copyright (C) 2000 Elsevier Science B.V.

AB - The CDC37 gene was isolated from a round-spotted pufferfish genomic library and characterized. This gene is composed of nine exons spanning 3.5 kb. Exon 1 contains the 5'-untranslated region and exon 2 contains the putative translation initiation site. By 5'-RACE (rapid amplication of cDNA ends) and sequence analysis, we deduced the promoter region for the CDC37 gene and found that it does not contain typical TATA or CCAAT box. The 1.8 kb DNA fragment upstream of the putative transcription initiation site contains numerous potential binding sites for transcription factors including CREB, E2A, Ets-1, GATA, NF-IL6 and PEA3. When this DNA fragment was placed upstream of the chloramphenicol acetyltransferase (CAT) reporter gene and transfected into a carp CF cell line, it could drive the synthesis of CAT enzyme four times more efficiently than the promoterless pCAT-Basic did. In addition, the CDC37 gene is linked to the TYK2 gene in a tail-to-head manner with a small intergenic region of 292 bp. Copyright (C) 2000 Elsevier Science B.V.

KW - CDC37

KW - Genomic structure

KW - Promoter

KW - Round-spotted pufferfish (Tetraodon fluviatilis)

UR - http://www.scopus.com/inward/record.url?scp=0034670139&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034670139&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(00)00138-X

DO - 10.1016/S0167-4781(00)00138-X

M3 - Article

C2 - 11072077

AN - SCOPUS:0034670139

VL - 1494

SP - 144

EP - 148

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 1-2

ER -