Genetic evidence for a contribution of EphA

EphrinA reverse signaling to motor axon guidance

Irina Dudanova, Tzu Jen Kao, Julia E. Herrmann, Binhai Zheng, Artur Kania, Rüdiger Klein

研究成果: 雜誌貢獻文章

32 引文 (Scopus)

摘要

Repulsive Eph forward signaling from limb-derived ephrins guides the axons of lateral motor column (LMC) motor neurons. LMC axons also express ephrinAs, while theirEphAreceptors are expressed in the limb mesenchyme. In vitro studies have suggested that reverse signaling from limb-derived EphA4 to axonal ephrinAs might result in attraction ofLMCaxons. However, genetic evidence for this function is lacking. Here we use theDunnchamber turning assay to show thatEphAproteins are chemoattractants and elicit fast turning responses inLMCneurons in vitro. Moreover, ectopic expression ofEphA4in chick hindlimb changes the limb trajectory ofLMCaxons. Nervous system-specific deletion ofEphA4 in mice resulted in fewer LMC axon projection errors than the ubiquitous deletion of EphA4. Additionally, a signaling-incompetent EphA4 mutant partially rescued guidance errors in the hindlimb, suggesting that limb-derived EphA4 contributes to the establishment ofLMCprojections. In summary, we provide evidence for a role of EphA:ephrinA attractive reverse signaling in motor axon guidance and in vivo evidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population.
原文英語
頁(從 - 到)5209-5215
頁數7
期刊Journal of Neuroscience
32
發行號15
DOIs
出版狀態已發佈 - 四月 11 2012
對外發佈Yes

指紋

Extremities
Ephrins
Axons
Hindlimb
Chemotactic Factors
Motor Neurons
Mesoderm
Nervous System
Axon Guidance
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Genetic evidence for a contribution of EphA : EphrinA reverse signaling to motor axon guidance. / Dudanova, Irina; Kao, Tzu Jen; Herrmann, Julia E.; Zheng, Binhai; Kania, Artur; Klein, Rüdiger.

於: Journal of Neuroscience, 卷 32, 編號 15, 11.04.2012, p. 5209-5215.

研究成果: 雜誌貢獻文章

Dudanova, Irina ; Kao, Tzu Jen ; Herrmann, Julia E. ; Zheng, Binhai ; Kania, Artur ; Klein, Rüdiger. / Genetic evidence for a contribution of EphA : EphrinA reverse signaling to motor axon guidance. 於: Journal of Neuroscience. 2012 ; 卷 32, 編號 15. 頁 5209-5215.
@article{0f1a70c5d79841d6b017ef6432dfaa76,
title = "Genetic evidence for a contribution of EphA: EphrinA reverse signaling to motor axon guidance",
abstract = "Repulsive Eph forward signaling from limb-derived ephrins guides the axons of lateral motor column (LMC) motor neurons. LMC axons also express ephrinAs, while theirEphAreceptors are expressed in the limb mesenchyme. In vitro studies have suggested that reverse signaling from limb-derived EphA4 to axonal ephrinAs might result in attraction ofLMCaxons. However, genetic evidence for this function is lacking. Here we use theDunnchamber turning assay to show thatEphAproteins are chemoattractants and elicit fast turning responses inLMCneurons in vitro. Moreover, ectopic expression ofEphA4in chick hindlimb changes the limb trajectory ofLMCaxons. Nervous system-specific deletion ofEphA4 in mice resulted in fewer LMC axon projection errors than the ubiquitous deletion of EphA4. Additionally, a signaling-incompetent EphA4 mutant partially rescued guidance errors in the hindlimb, suggesting that limb-derived EphA4 contributes to the establishment ofLMCprojections. In summary, we provide evidence for a role of EphA:ephrinA attractive reverse signaling in motor axon guidance and in vivo evidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population.",
author = "Irina Dudanova and Kao, {Tzu Jen} and Herrmann, {Julia E.} and Binhai Zheng and Artur Kania and R{\"u}diger Klein",
year = "2012",
month = "4",
day = "11",
doi = "10.1523/JNEUROSCI.5707-11.2012",
language = "English",
volume = "32",
pages = "5209--5215",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "15",

}

TY - JOUR

T1 - Genetic evidence for a contribution of EphA

T2 - EphrinA reverse signaling to motor axon guidance

AU - Dudanova, Irina

AU - Kao, Tzu Jen

AU - Herrmann, Julia E.

AU - Zheng, Binhai

AU - Kania, Artur

AU - Klein, Rüdiger

PY - 2012/4/11

Y1 - 2012/4/11

N2 - Repulsive Eph forward signaling from limb-derived ephrins guides the axons of lateral motor column (LMC) motor neurons. LMC axons also express ephrinAs, while theirEphAreceptors are expressed in the limb mesenchyme. In vitro studies have suggested that reverse signaling from limb-derived EphA4 to axonal ephrinAs might result in attraction ofLMCaxons. However, genetic evidence for this function is lacking. Here we use theDunnchamber turning assay to show thatEphAproteins are chemoattractants and elicit fast turning responses inLMCneurons in vitro. Moreover, ectopic expression ofEphA4in chick hindlimb changes the limb trajectory ofLMCaxons. Nervous system-specific deletion ofEphA4 in mice resulted in fewer LMC axon projection errors than the ubiquitous deletion of EphA4. Additionally, a signaling-incompetent EphA4 mutant partially rescued guidance errors in the hindlimb, suggesting that limb-derived EphA4 contributes to the establishment ofLMCprojections. In summary, we provide evidence for a role of EphA:ephrinA attractive reverse signaling in motor axon guidance and in vivo evidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population.

AB - Repulsive Eph forward signaling from limb-derived ephrins guides the axons of lateral motor column (LMC) motor neurons. LMC axons also express ephrinAs, while theirEphAreceptors are expressed in the limb mesenchyme. In vitro studies have suggested that reverse signaling from limb-derived EphA4 to axonal ephrinAs might result in attraction ofLMCaxons. However, genetic evidence for this function is lacking. Here we use theDunnchamber turning assay to show thatEphAproteins are chemoattractants and elicit fast turning responses inLMCneurons in vitro. Moreover, ectopic expression ofEphA4in chick hindlimb changes the limb trajectory ofLMCaxons. Nervous system-specific deletion ofEphA4 in mice resulted in fewer LMC axon projection errors than the ubiquitous deletion of EphA4. Additionally, a signaling-incompetent EphA4 mutant partially rescued guidance errors in the hindlimb, suggesting that limb-derived EphA4 contributes to the establishment ofLMCprojections. In summary, we provide evidence for a role of EphA:ephrinA attractive reverse signaling in motor axon guidance and in vivo evidence of in-parallel forward Eph and reverse ephrin signaling function in the same neuronal population.

UR - http://www.scopus.com/inward/record.url?scp=84859524688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859524688&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.5707-11.2012

DO - 10.1523/JNEUROSCI.5707-11.2012

M3 - Article

VL - 32

SP - 5209

EP - 5215

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 15

ER -