Genetic analysis of enterovirus 71 isolated from fatal and non-fatal cases of hand, foot and mouth disease during an epidemic in Taiwan, 1998

Shin Ru Shih, Mei Shang Ho, Kuei Hsiang Lin, Shu Li Wu, Yen Tsun Chen, Cheng Nan Wu, Tzou Yien Lin, Luan Yin Chang, Kuo Chien Tsao, Hsiao Chen Ning, Pi Yueh Chang, Shih Ming Jung, Chuen Hsueh, Kenneth S. Chang

研究成果: 雜誌貢獻文章同行評審

123 引文 斯高帕斯(Scopus)

摘要

A large scale outbreak of hand-foot-and-mouth disease (HFMD) occurred in Taiwan in 1998, in which more than 80 children died of shock syndrome with pulmonary edema/hemorrhage. Enterovirus 71 was implicated as the cause of this outbreak. In order to understand the virological basis responsible for mortality on this scale, nucleotide sequences of VP1 that is important for serotypic specificity, and the 5'-non-coding region (5'-NCR) that is important for replication efficiency, were analyzed comparatively. Phylogenetic analysis of both VP1 and 5'-NCR of nine EV71 isolates derived from specimens of fatal patients and seven isolates derived from uncomplicated HFMD patients showed that all but one isolate fell into genotype B. The one distinct isolate from a case of uncomplicated HFMD belonged to genotype C that was clustered along with one isolate from Taiwan in 1986. Complete sequence analysis of two selected isolates, one from the spinal cord of a fatal case and one from the vesicle fluid of a patient with mild HFMD, confirmed a high degree (97-100%) of identity in nucleotide sequence throughout the entire genome, except focal regions of 3C and 3'-NCR where the nucleotide homology was 90-91%. The identity of the deduced amino acid sequence in the 3C region that encodes viral proteinase dropped further to 86%, a result of missense mutations at the first nucleotide position of many codons. (C) 2000 Elsevier Science B.V.
原文英語
頁(從 - 到)127-136
頁數10
期刊Virus Research
68
發行號2
DOIs
出版狀態已發佈 - 七月 10 2000
對外發佈Yes

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

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