Generation and characterization of anti-α-enolase single-chain antibodies in chicken

Sy Jye Leu, Yu Ching Lee, Neng Yao Shih, I. Jen Huang, Ko Jiunn Liu, Hsu Feng Lu, Shih Yi Huang, Yi Yuan Yang

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

It was previously reported that up-regulation of α-enolase protein was detected in 65% of patients with non-small cell lung cancers (NSCLC). Moreover, a high titer of anti-α-enolase antibodies was developed in a smaller proportion (7.4%) of these patients than in non-tumor-associated patients and healthy subjects. In the present study, we characterized polyclonal and single-chain variable fragment (scFv) anti-α-enolase antibodies from immunized chickens. The E. coli-derived recombinant α-enolase protein was purified to its high homogenicity as verified by SDS-PAGE. After the 4th immunization, a high titer of specific polyclonal anti-α-enolase antibodies was elicited in immunized chickens and specifically recognized the purified human α-enolase antigen as determined by Western blot and ELISA. The expressed heavy and light chain variable genes (VH and VL) were isolated from spleen B cells and amplified to construct phage antibody libraries containing scFv molecules. After four rounds of panning selection, the scFv antibodies of randomly chosen clones were expressed and their binding specificity to α-enolase protein was verified using competitive ELISA, flow cytometry and immunofluorescence staining. Nucleotide sequence analysis from 10 α-enolase binding clones showed that 3 (30%) clones used identical heavy and light genes for scFv antibody expression, as represented by EnL5. Notably, amino acid changes in complementarity-determining regions (CDRs) were more frequently observed than those in framework regions (FRs) in all clones, indicating a strong affinity selection through mutations. All together, it is believed that these polyclonal and scFv IgY antibodies may be helpful in the development of molecular diagnostic and therapeutic agents for lung cancers in the future.

原文英語
頁(從 - 到)251-260
頁數10
期刊Veterinary Immunology and Immunopathology
137
發行號3-4
DOIs
出版狀態已發佈 - 十月 2010

指紋

Single-Chain Antibodies
phosphopyruvate hydratase
Phosphopyruvate Hydratase
Chickens
chickens
antibodies
Clone Cells
clones
Anti-Idiotypic Antibodies
lung neoplasms
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Complementarity Determining Regions
Light
Molecular Pathology
proteins
Recombinant Proteins
Non-Small Cell Lung Carcinoma
bacteriophages
Bacteriophages

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

引用此文

Generation and characterization of anti-α-enolase single-chain antibodies in chicken. / Leu, Sy Jye; Lee, Yu Ching; Shih, Neng Yao; Huang, I. Jen; Liu, Ko Jiunn; Lu, Hsu Feng; Huang, Shih Yi; Yang, Yi Yuan.

於: Veterinary Immunology and Immunopathology, 卷 137, 編號 3-4, 10.2010, p. 251-260.

研究成果: 雜誌貢獻文章

Leu, Sy Jye ; Lee, Yu Ching ; Shih, Neng Yao ; Huang, I. Jen ; Liu, Ko Jiunn ; Lu, Hsu Feng ; Huang, Shih Yi ; Yang, Yi Yuan. / Generation and characterization of anti-α-enolase single-chain antibodies in chicken. 於: Veterinary Immunology and Immunopathology. 2010 ; 卷 137, 編號 3-4. 頁 251-260.
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abstract = "It was previously reported that up-regulation of α-enolase protein was detected in 65{\%} of patients with non-small cell lung cancers (NSCLC). Moreover, a high titer of anti-α-enolase antibodies was developed in a smaller proportion (7.4{\%}) of these patients than in non-tumor-associated patients and healthy subjects. In the present study, we characterized polyclonal and single-chain variable fragment (scFv) anti-α-enolase antibodies from immunized chickens. The E. coli-derived recombinant α-enolase protein was purified to its high homogenicity as verified by SDS-PAGE. After the 4th immunization, a high titer of specific polyclonal anti-α-enolase antibodies was elicited in immunized chickens and specifically recognized the purified human α-enolase antigen as determined by Western blot and ELISA. The expressed heavy and light chain variable genes (VH and VL) were isolated from spleen B cells and amplified to construct phage antibody libraries containing scFv molecules. After four rounds of panning selection, the scFv antibodies of randomly chosen clones were expressed and their binding specificity to α-enolase protein was verified using competitive ELISA, flow cytometry and immunofluorescence staining. Nucleotide sequence analysis from 10 α-enolase binding clones showed that 3 (30{\%}) clones used identical heavy and light genes for scFv antibody expression, as represented by EnL5. Notably, amino acid changes in complementarity-determining regions (CDRs) were more frequently observed than those in framework regions (FRs) in all clones, indicating a strong affinity selection through mutations. All together, it is believed that these polyclonal and scFv IgY antibodies may be helpful in the development of molecular diagnostic and therapeutic agents for lung cancers in the future.",
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AU - Lu, Hsu Feng

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