Gene treatment of cerebral stroke by rAAV vector delivering IL-Ira in a rat model

Tung Han Tsai, Show Li Chen, Xiao Xiao, Yung Hsiao Chiang, Shinn Zong Lin, Shu Wen Kuo, Dai Wei Liu, Yeou Ping Tsao

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

In this study, we injected recombinant adeno-associated virus (rAAV) vectors expressing the interleukin-I receptor antagonist (rAAV-IL-Ira) into the cortex of rats experiencing transient cerebral ischemia. An accumulation of IL-Ira in cortical tissues of rAAV-IL-Ira-injected animals was confirmed by ELISA. Triphenyltetrazolium chloride (TTC) staining of viable brain tissue revealed that the rAAV-delivered IL-Ira gene could rescue the brain tissues from ischemia-induced injury. Cortical tissues that received rAAV-IL-Ira injections had both significantly smaller total volumes of infarction as well as smaller areas of infarction on each brain slice when compared with the control models. In situ labeling analysis demonstrated significant reduction of apoptotic cells in cortical tissues rescued by rAAV-IL-Ira. Immunohistochemistry staining revealed that the rescued brain tissues contained the same levels of neuronal cells as contralateral undamaged brain tissues. These findings confirmed that the rAAV delivering the IL-Ira gene is a potential therapy for stroke.

原文英語
頁(從 - 到)803-807
頁數5
期刊NeuroReport
14
發行號6
DOIs
出版狀態已發佈 - 五月 6 2003
對外發佈Yes

指紋

Dependovirus
Stroke
Genes
Brain
Infarction
Therapeutics
Interleukin Receptors
Staining and Labeling
Transient Ischemic Attack
Brain Ischemia
Interleukin-1
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Injections
Wounds and Injuries

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Tsai, T. H., Chen, S. L., Xiao, X., Chiang, Y. H., Lin, S. Z., Kuo, S. W., ... Tsao, Y. P. (2003). Gene treatment of cerebral stroke by rAAV vector delivering IL-Ira in a rat model. NeuroReport, 14(6), 803-807. https://doi.org/10.1097/00001756-200305060-00005

Gene treatment of cerebral stroke by rAAV vector delivering IL-Ira in a rat model. / Tsai, Tung Han; Chen, Show Li; Xiao, Xiao; Chiang, Yung Hsiao; Lin, Shinn Zong; Kuo, Shu Wen; Liu, Dai Wei; Tsao, Yeou Ping.

於: NeuroReport, 卷 14, 編號 6, 06.05.2003, p. 803-807.

研究成果: 雜誌貢獻文章

Tsai, TH, Chen, SL, Xiao, X, Chiang, YH, Lin, SZ, Kuo, SW, Liu, DW & Tsao, YP 2003, 'Gene treatment of cerebral stroke by rAAV vector delivering IL-Ira in a rat model', NeuroReport, 卷 14, 編號 6, 頁 803-807. https://doi.org/10.1097/00001756-200305060-00005
Tsai, Tung Han ; Chen, Show Li ; Xiao, Xiao ; Chiang, Yung Hsiao ; Lin, Shinn Zong ; Kuo, Shu Wen ; Liu, Dai Wei ; Tsao, Yeou Ping. / Gene treatment of cerebral stroke by rAAV vector delivering IL-Ira in a rat model. 於: NeuroReport. 2003 ; 卷 14, 編號 6. 頁 803-807.
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