Gene-to-gene network analysis of the mediation of plant innate immunity by the eliciting plant response-like 1 (Epl1) elicitor of trichoderma formosa

Chi Hua Cheng, Bing Nan Shen, Qian Wen Shang, Li Yu Daisy Liu, Kou Cheng Peng, Yan Huey Chen, Fang Fang Chen, Sin Fen Hu, Yu Tai Wang, Hao Ching Wang, Hsin Yi Wu, Chaur Tsuen Lo, Shih Shun Lin

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

A new clade, Trichoderma formosa, secretes eliciting plant response-like 1 (Epl1), a small peptide elicitor that stimulates plant immunity. Nicotiana benthamiana pretreated with Epl1 for 3 days developed immunity against Tomato mosaic virus (ToMV) infection. The transcriptome profiles of T. formosa and N. benthamiana were obtained by deep sequencing; the transcript of Epl1 is 736 nt in length and encodes a 12-kDa peptide. Identifying critical genes in Epl1-mediated immunity was challenging due to high similarity between the transcriptome expression profiles of Epl1-treated and ToMV-infected N. benthamiana samples. Therefore, an efficient bioinformatics data mining approach was used for high-throughput transcriptomic assays in this study. We integrated gene-to-gene network analysis into the ContigViews transcriptome database, and genes related to jasmonic acid and ethylene signaling, salicylic acid signaling, leucine-rich repeats, transcription factors, and histone variants were hubs in the gene-to-gene networks. In this study, the Epl1 of T. formosa triggers plant immunity against various pathogen infections. Moreover, we demonstrated that high-throughput data mining and gene-to-gene network analysis can be used to identify critical candidate genes for further studies on the mechanisms of plant immunity.
原文英語
頁(從 - 到)683-691
頁數9
期刊Molecular Plant-Microbe Interactions
31
發行號7
DOIs
出版狀態已發佈 - 七月 1 2018

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

指紋 深入研究「Gene-to-gene network analysis of the mediation of plant innate immunity by the eliciting plant response-like 1 (Epl1) elicitor of trichoderma formosa」主題。共同形成了獨特的指紋。

引用此