Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

Nobuyuki Yamamichi, Kamolparn Pugdee, Wei Jen Chang, Sheng Yang Lee, Masao Yoshinari, Tohru Hayakawa, Yoshimitsu Abiko

研究成果: 雜誌貢獻文章

37 引文 斯高帕斯(Scopus)

摘要

Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.
原文英語
頁(從 - 到)744-750
頁數7
期刊Dental Materials Journal
27
發行號5
DOIs
出版狀態已發佈 - 2008

ASJC Scopus subject areas

  • Dentistry(all)
  • Ceramics and Composites

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