Gene expression and behavior analysis of PC12 cells grown on biodegradable nano-fibrous membranes

Shwu Fen Chang, Chih Ping Yang, Chien Cheng Tai, How Tseng

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

The use of biodegradable synthetic polyesters as peripheral nerve conduits and the "contact guidance" effect of nano-sized fibrous matrices on neurite outgrowth have been reported; however, no literature exists regarding how these matrices regulate neurite growth-related gene expression. Such information would be useful in the evaluation of the biological applicability of various materials. In this study, two biodegradable synthetic polyesters (PLLA and PBSA) were employed to fabricate a series of micro/nano-fibrous membranes that were used as culture matrices for the in vitro growth of PC12 cells in response to nerve growth factor (NGF). Cell adhesion, proliferation, and differentiation were analyzed in addition to neurite outgrowth- and apoptosis-related gene expression. Our results show that cells grown on electrospun PBSA membranes proliferate to a greater extent than cells cultured on tissue culture polystyrene (TCPS) and other prepared membranes. Compared to TCPS and PBSA, growth on an electrospun PLLA membrane triggers higher GAP43 and MAP2 gene expression in PC12 cells. No significant effect of membrane type on apoptosis-related gene expression was observed. These results indicate that electrospun nano-fibrous PLLA mats are more suitable for use as nerve conduit matrices than conventional non/micro-fibrous PLLA or PBSA membranes.

原文英語
頁(從 - 到)886-892
頁數7
期刊Current Nanoscience
7
發行號6
DOIs
出版狀態已發佈 - 十二月 2011

指紋

Fibrous membranes
PC12 Cells
Gene expression
Cells
Membranes
Gene Expression
Tissue culture
Polyesters
Polystyrenes
Cell death
Apoptosis
Growth
Cell adhesion
Nerve Growth Factor
Neurites
Peripheral Nerves
Cell Adhesion
Cell Differentiation
Cultured Cells
Cell Proliferation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

引用此文

Gene expression and behavior analysis of PC12 cells grown on biodegradable nano-fibrous membranes. / Chang, Shwu Fen; Yang, Chih Ping; Tai, Chien Cheng; Tseng, How.

於: Current Nanoscience, 卷 7, 編號 6, 12.2011, p. 886-892.

研究成果: 雜誌貢獻文章

@article{75b6aa7807d348a9bc4c445f352cdb4f,
title = "Gene expression and behavior analysis of PC12 cells grown on biodegradable nano-fibrous membranes",
abstract = "The use of biodegradable synthetic polyesters as peripheral nerve conduits and the {"}contact guidance{"} effect of nano-sized fibrous matrices on neurite outgrowth have been reported; however, no literature exists regarding how these matrices regulate neurite growth-related gene expression. Such information would be useful in the evaluation of the biological applicability of various materials. In this study, two biodegradable synthetic polyesters (PLLA and PBSA) were employed to fabricate a series of micro/nano-fibrous membranes that were used as culture matrices for the in vitro growth of PC12 cells in response to nerve growth factor (NGF). Cell adhesion, proliferation, and differentiation were analyzed in addition to neurite outgrowth- and apoptosis-related gene expression. Our results show that cells grown on electrospun PBSA membranes proliferate to a greater extent than cells cultured on tissue culture polystyrene (TCPS) and other prepared membranes. Compared to TCPS and PBSA, growth on an electrospun PLLA membrane triggers higher GAP43 and MAP2 gene expression in PC12 cells. No significant effect of membrane type on apoptosis-related gene expression was observed. These results indicate that electrospun nano-fibrous PLLA mats are more suitable for use as nerve conduit matrices than conventional non/micro-fibrous PLLA or PBSA membranes.",
keywords = "Electrospinning, Neurite outgrowth, PBSA, PC12 cells, PLLA",
author = "Chang, {Shwu Fen} and Yang, {Chih Ping} and Tai, {Chien Cheng} and How Tseng",
year = "2011",
month = "12",
doi = "10.2174/157341311798220547",
language = "English",
volume = "7",
pages = "886--892",
journal = "Current Nanoscience",
issn = "1573-4137",
publisher = "Bentham Science Publishers B.V.",
number = "6",

}

TY - JOUR

T1 - Gene expression and behavior analysis of PC12 cells grown on biodegradable nano-fibrous membranes

AU - Chang, Shwu Fen

AU - Yang, Chih Ping

AU - Tai, Chien Cheng

AU - Tseng, How

PY - 2011/12

Y1 - 2011/12

N2 - The use of biodegradable synthetic polyesters as peripheral nerve conduits and the "contact guidance" effect of nano-sized fibrous matrices on neurite outgrowth have been reported; however, no literature exists regarding how these matrices regulate neurite growth-related gene expression. Such information would be useful in the evaluation of the biological applicability of various materials. In this study, two biodegradable synthetic polyesters (PLLA and PBSA) were employed to fabricate a series of micro/nano-fibrous membranes that were used as culture matrices for the in vitro growth of PC12 cells in response to nerve growth factor (NGF). Cell adhesion, proliferation, and differentiation were analyzed in addition to neurite outgrowth- and apoptosis-related gene expression. Our results show that cells grown on electrospun PBSA membranes proliferate to a greater extent than cells cultured on tissue culture polystyrene (TCPS) and other prepared membranes. Compared to TCPS and PBSA, growth on an electrospun PLLA membrane triggers higher GAP43 and MAP2 gene expression in PC12 cells. No significant effect of membrane type on apoptosis-related gene expression was observed. These results indicate that electrospun nano-fibrous PLLA mats are more suitable for use as nerve conduit matrices than conventional non/micro-fibrous PLLA or PBSA membranes.

AB - The use of biodegradable synthetic polyesters as peripheral nerve conduits and the "contact guidance" effect of nano-sized fibrous matrices on neurite outgrowth have been reported; however, no literature exists regarding how these matrices regulate neurite growth-related gene expression. Such information would be useful in the evaluation of the biological applicability of various materials. In this study, two biodegradable synthetic polyesters (PLLA and PBSA) were employed to fabricate a series of micro/nano-fibrous membranes that were used as culture matrices for the in vitro growth of PC12 cells in response to nerve growth factor (NGF). Cell adhesion, proliferation, and differentiation were analyzed in addition to neurite outgrowth- and apoptosis-related gene expression. Our results show that cells grown on electrospun PBSA membranes proliferate to a greater extent than cells cultured on tissue culture polystyrene (TCPS) and other prepared membranes. Compared to TCPS and PBSA, growth on an electrospun PLLA membrane triggers higher GAP43 and MAP2 gene expression in PC12 cells. No significant effect of membrane type on apoptosis-related gene expression was observed. These results indicate that electrospun nano-fibrous PLLA mats are more suitable for use as nerve conduit matrices than conventional non/micro-fibrous PLLA or PBSA membranes.

KW - Electrospinning

KW - Neurite outgrowth

KW - PBSA

KW - PC12 cells

KW - PLLA

UR - http://www.scopus.com/inward/record.url?scp=82155170732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82155170732&partnerID=8YFLogxK

U2 - 10.2174/157341311798220547

DO - 10.2174/157341311798220547

M3 - Article

AN - SCOPUS:82155170732

VL - 7

SP - 886

EP - 892

JO - Current Nanoscience

JF - Current Nanoscience

SN - 1573-4137

IS - 6

ER -