Gene and protein expressions of bone marrow mesenchymal stem cells in a bone tunnel for tendon-bone healing

Chih Hwa Chen, Shu Wen Whu, Chih Hsiang Chang, Chun I. Su

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Purpose: Bone marrow mesenchymal stem cells (BMSCs) injected around tendon grafts can enhance tendon-bone healing and promote fibrocartilage formation. To understand gene and protein expressions of cells during tendon-bone healing, auto-BMSCs were implanted into a bone tunnel in anterior cruciate ligament reconstruction in a rabbit model. Methods: BMSCs were harvested from New Zealand white rabbits. By an anterior cruciate ligament reconstruction model, 1×106 BMSCs in 0.35mL of fibrin glue was injected into bone tunnel as Fibrin-BMSC group. Only fibrin glue (Fibrin group) was injected as control. Three chondrogenesis genes and proteins, including Sox 9, collagen Type II (COII), aggrecan, and three osteogenesis genes and proteins, including Runx2, collagen type I (COI), and osteocalcin, between Fibrin-BMSC and Fibrin group were compared by real-time polymerase chain reaction assay and immunohistochemical assay postoperation. Results: In real-time polymerase chain reaction assay, Sox9, COII, aggrecan, COI, and osteocalcin expressions upregulated and Runx2 downregulated were determined in Fibrin-BMSC group at 1 week. COII, aggrecan upregulated, and Runx2 and osteocalcin downregulated were determined at 4 weeks. In immunohistochemical assay, only Sox9, COII, and aggrecan proteins in only Fibrin-BMSC group were observed at 4 weeks. The protein expression as same as gene expression was obtained in a bone tunnel. Conclusion: Auto-BMSCs promoted COII and aggrecan expression and reduced Runx2 and osteocalcin expression in a bone tunnel. It demonstrated that these cells could enhance fibrocartilage formation because of higher chondrogenesis expression during tendon-bone healing.

原文英語
頁(從 - 到)85-93
頁數9
期刊Formosan Journal of Musculoskeletal Disorders
2
發行號3
DOIs
出版狀態已發佈 - 八月 2011
對外發佈

ASJC Scopus subject areas

  • 骨科和運動醫學

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