Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing

Yu Chih Huang, Kuen Yu Huang, Wei Zhen Lew, Kang Hsin Fan, Wei Jen Chang, Haw Ming Huang

研究成果: 雜誌貢獻文章

摘要

In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ -irradiation. The chemical and physical properties of γ -irradiated LMWHA and the in vitro cellular growth experiments with γ -irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ -irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ -irradiated LMWHA demonstrated a significant alteration in carbon-oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ -irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ -irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ -irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ -irradiated LMWHA could be a potential source for the promotion of skin wound healing.

原文英語
文章編號1214
期刊Polymers
11
發行號7
DOIs
出版狀態已發佈 - 七月 1 2019

指紋

Hyaluronic acid
Hyaluronic Acid
Skin
Molecular weight
Irradiation
Carboxymethylcellulose Sodium
Fibroblasts
Cell culture
Powders
Chemical properties
Strain rate
Cellulose
Animals
Carbon
Physical properties
Experiments
Nuclear magnetic resonance
Viscosity
Oxygen
Fluids

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

引用此文

Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing. / Huang, Yu Chih; Huang, Kuen Yu; Lew, Wei Zhen; Fan, Kang Hsin; Chang, Wei Jen; Huang, Haw Ming.

於: Polymers, 卷 11, 編號 7, 1214, 01.07.2019.

研究成果: 雜誌貢獻文章

Huang, Yu Chih ; Huang, Kuen Yu ; Lew, Wei Zhen ; Fan, Kang Hsin ; Chang, Wei Jen ; Huang, Haw Ming. / Gamma-irradiation-prepared low molecular weight hyaluronic acid promotes skin wound healing. 於: Polymers. 2019 ; 卷 11, 編號 7.
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