Gadolinium hexanedione nanoparticles for stem cell labeling and tracking via magnetic resonance imaging

Ching L. Tseng, I. Ling Shih, Leszek Stobinski, Feng Huei Lin

研究成果: 雜誌貢獻文章

50 引文 (Scopus)

摘要

The ability to trace transplanted stem cells and monitor their tissue biodistribution is prerequisite to an understanding of cellular migration after transplantation. Therefore, a new magnetic resonance imaging (MRI) contrast agent made of gadolinium hexanedione nanoparticles (GdH-NPs) was developed as a cell tracking agent. The GdH-NPs were fabricated by the microemulsion process. The physical characteristics, biocompatibility, and T1-MRI signal enhancement of these NPs were analyzed and evaluated for stem cell tracking. In this study, the size of the synthesized GdH-NPs was about 140. nm, and it had greater image enhancement ability than commercial gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). From the biocompability test, we found GdH-NPs were nontoxic for human mesenchymal stem cells (hMSCs). The expression of surface antigens of hMSCs after culture with GdH-NPs was examined, and it showed no difference from the control group. The results of transmission electron microscopy (TEM) imaging for labeled hMSCs showed GdH-NPs were accumulated in the cells by the endocytotic pathway. The accumulation of GdH-NPs in hMSCs was three times higher in comparison to Gd-DTPA. Human MSCs labeled with low concentration of GdH-NPs (10μg/mL) hold better signals in cellular MR image. We conclude GdH-NPs can be used to label hMSCs in vitro with greater T1 image-enhancing property and without affecting cell quality. Finally, GdH-NPs have great potential as a contrast agent for stem cell tracking by MRI methodology.
原文英語
頁(從 - 到)5427-5435
頁數9
期刊Biomaterials
31
發行號20
DOIs
出版狀態已發佈 - 七月 2010
對外發佈Yes

指紋

Cell Tracking
Gadolinium
Magnetic resonance
Stem cells
Nanoparticles
Labeling
Stem Cells
Magnetic Resonance Imaging
Imaging techniques
Mesenchymal Stromal Cells
Pentetic Acid
Contrast Media
gadolinium hexanedione
Image Enhancement
Image enhancement
Microemulsions
Surface Antigens
Transmission Electron Microscopy
Biocompatibility
Cell culture

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites

引用此文

Gadolinium hexanedione nanoparticles for stem cell labeling and tracking via magnetic resonance imaging. / Tseng, Ching L.; Shih, I. Ling; Stobinski, Leszek; Lin, Feng Huei.

於: Biomaterials, 卷 31, 編號 20, 07.2010, p. 5427-5435.

研究成果: 雜誌貢獻文章

Tseng, Ching L. ; Shih, I. Ling ; Stobinski, Leszek ; Lin, Feng Huei. / Gadolinium hexanedione nanoparticles for stem cell labeling and tracking via magnetic resonance imaging. 於: Biomaterials. 2010 ; 卷 31, 編號 20. 頁 5427-5435.
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