TY - JOUR
T1 - GABA tea attenuates cardiac apoptosis in spontaneously hypertensive rats (SHR) by enhancing PI3K/Akt-mediated survival pathway and suppressing Bax/Bak dependent apoptotic pathway
AU - Chen, Bih Cheng
AU - Hung, Meng Yu
AU - Wang, Hsueh Fang
AU - Yeh, Li Jen
AU - Pandey, Sudhir
AU - Chen, Ray Jade
AU - Chang, Ruey Lin
AU - Viswanadha, Vijaya Padma
AU - Lin, Kuan Ho
AU - Huang, Chih Yang
N1 - Funding Information:
This study was supported by Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence MOHW106-TDU-B-212?113004.
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2018/7
Y1 - 2018/7
N2 - Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male SHR at eight weeks of age, 200 g were divided into six groups. One group was fed water as a control group. Other rats were administered one of the following treatments: GABA tea at dose 150 and 300 mg/kg/day as low GABA tea (LGT) and high GABA tea (HGT) groups, respectively, pure GABA at dose 150 and 300 mg/kg/day as LG and HG groups, respectively, green tea (GT) as control of LGT and HGT groups. After 12 weeks, cardiac tissues were analyzed by histological analysis, western blotting, and TUNEL assays. GABA tea, GT, and pure GABA decreased hypertension-induced cardiac abnormalities, including abnormal myocardial architecture. In addition, GABA tea, GT, and pure GABA dramatically increased anti-apoptotic protein, Bcl2. Furthermore, GABA tea, GT, and pure GABA also decreased activated-caspase 9 and activated-caspase 3. Additionally, the survival associated protein IGF-I and PI3K/Akt were enhanced in cardiac tissues upon treatment. Our results showed an optimistic anti-apoptotic and pro-survival effects of GABA tea treatment against hypertensive rat hearts.
AB - Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male SHR at eight weeks of age, 200 g were divided into six groups. One group was fed water as a control group. Other rats were administered one of the following treatments: GABA tea at dose 150 and 300 mg/kg/day as low GABA tea (LGT) and high GABA tea (HGT) groups, respectively, pure GABA at dose 150 and 300 mg/kg/day as LG and HG groups, respectively, green tea (GT) as control of LGT and HGT groups. After 12 weeks, cardiac tissues were analyzed by histological analysis, western blotting, and TUNEL assays. GABA tea, GT, and pure GABA decreased hypertension-induced cardiac abnormalities, including abnormal myocardial architecture. In addition, GABA tea, GT, and pure GABA dramatically increased anti-apoptotic protein, Bcl2. Furthermore, GABA tea, GT, and pure GABA also decreased activated-caspase 9 and activated-caspase 3. Additionally, the survival associated protein IGF-I and PI3K/Akt were enhanced in cardiac tissues upon treatment. Our results showed an optimistic anti-apoptotic and pro-survival effects of GABA tea treatment against hypertensive rat hearts.
KW - Apoptosis
KW - Bak
KW - Bax
KW - BCL2
KW - Cardiac hypertension
KW - GABA tea
KW - P-Akt
KW - p-Akt
KW - cardiac hypertension
KW - apoptosis
KW - Caspase 3/metabolism
KW - Phosphatidylinositol 3-Kinases/metabolism
KW - Male
KW - Proto-Oncogene Proteins c-akt/metabolism
KW - Insulin-Like Growth Factor I/metabolism
KW - bcl-2-Associated X Protein/metabolism
KW - bcl-2 Homologous Antagonist-Killer Protein/metabolism
KW - Hypertension/drug therapy
KW - Rats, Inbred SHR
KW - Apoptosis/drug effects
KW - Rats
KW - Signal Transduction/drug effects
KW - Heart Ventricles/drug effects
KW - Animals
KW - Receptors, Somatomedin/metabolism
KW - Tea/chemistry
KW - gamma-Aminobutyric Acid/pharmacology
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UR - http://www.scopus.com/inward/citedby.url?scp=85046069112&partnerID=8YFLogxK
U2 - 10.1002/tox.22565
DO - 10.1002/tox.22565
M3 - Article
C2 - 29708300
AN - SCOPUS:85046069112
VL - 33
SP - 789
EP - 797
JO - Environmental Toxicology
JF - Environmental Toxicology
SN - 1520-4081
IS - 7
ER -