Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow

Ming Song Tsai, Shiaw Min Hwang, Kuang Den Chen, Yun Shien Lee, Li Wen Hsu, Yu Jen Chang, Chao Nin Wang, Hsiu Huei Peng, Yao Lung Chang, An Shine Chao, Shuenn Dyh Chang, Kuan Der Lee, Tzu Hao Wang, Hsin Shih Wang, Yung Kuei Soong

研究成果: 雜誌貢獻文章同行評審

182 引文 斯高帕斯(Scopus)

摘要

Using high-density oligonucleotide microarrays and functional network analyses, we examined whether MSCs derived from four different origins exhibited unique gene expression profiles individually and then compared the gene expression profiles of all MSCs with those of fetal organs. Our results indicated that within each group of MSCs from the same origin, the variability of the gene expression levels was smaller than that between groups of different origins. Functional genomic studies revealed the specific roles of MSCs from different origins. Our results suggest that amniotic fluid MSCs may initiate interactions with the uterus by upregulating oxytocin and thrombin receptors. Amniotic membrane MSCs may play a role in maintaining homeostasis of fluid and electrolytes by regulating the networks of endothelin, neprilysin, bradykinin receptors, and atrial natriuretic peptide. Cord blood MSCs may be involved in innate immune systems as the neonatal defense system against the earliest encountered pathogens. Adult bone marrow MSCs may be an important source not only of all blood lineages but also of bone formation. However, in spite of the different gene expression profiles seen in MSCs derived from different origins, a set of core gene expression profiles was preserved in these four kinds of MSCs. The core signature transcriptomes of all MSCs, when contrasted against those of fetal organs, included genes involved in the regulation of extracellular matrix and adhesion, transforming growth factor-β receptor signaling, and the Wnt signaling pathways.
原文英語
頁(從 - 到)2511-2523
頁數13
期刊Stem Cells
25
發行號10
DOIs
出版狀態已發佈 - 十月 2007
對外發佈

ASJC Scopus subject areas

  • 分子醫學
  • 發展生物學
  • 細胞生物學

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