Functional domains of pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. coli

Jaulang Hwang, David J. Fitzgerald, Sankar Adhya, Ira Pastan

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336 引文 斯高帕斯(Scopus)

摘要

Pseudomonas exotoxin A is a single chain toxin with three structural domains that inhibits protein synthesis in eukaryotic cells by catalyzing ADP ribosylation of elongation factor 2. To study the function of these domains, we deleted different portions of the PE structural gene and expressed these constructs in E. coli using an inducible T7 promoter. These studies indicate that structural domain la is required for cell recognition, that structural domain ll is required to translocate the toxin across a cellular membrane, and that structural domain III and a portion of domain lb are required for ADP ribosylation activity. Toxin lacking domain la is about 100-fold less toxic to mice than intact PE and should be a useful molecule for the construction of immunotoxins.

原文英語
頁(從 - 到)129-136
頁數8
期刊Cell
48
發行號1
DOIs
出版狀態已發佈 - 一月 16 1987
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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