Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice

Chien Ming Chen, Yih Huei Uen, Chen Yi Kuo, Tzu Chuan Huang, Jen Ai Lee

研究成果: 書貢獻/報告類型會議貢獻

3 引文 (Scopus)

摘要

We determined the concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of normal and aristolochic acid (AA)-treated mice. The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically by column-switching high-performance liquid chromatography (HPLC). Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for enantiomeric separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p<0.05), suggesting that serum L-3HB may be used as an indicator to determine the level of renal damage.
原文英語
主出版物標題Communications in Computer and Information Science
頁面63-68
頁數6
57 CCIS
DOIs
出版狀態已發佈 - 2009
事件International Conference on Bio-Science and Bio-Technology, BSBT 2009. Held as Part of the Future Generation Information Technology Conference, FGIT 2009 - Jeju Island, 大韓民國
持續時間: 十二月 10 2009十二月 12 2009

出版系列

名字Communications in Computer and Information Science
57 CCIS
ISSN(列印)18650929

其他

其他International Conference on Bio-Science and Bio-Technology, BSBT 2009. Held as Part of the Future Generation Information Technology Conference, FGIT 2009
國家大韓民國
城市Jeju Island
期間12/10/0912/12/09

指紋

Acids
High performance liquid chromatography
Derivatives

ASJC Scopus subject areas

  • Computer Science(all)

引用此文

Chen, C. M., Uen, Y. H., Kuo, C. Y., Huang, T. C., & Lee, J. A. (2009). Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. 於 Communications in Computer and Information Science (卷 57 CCIS, 頁 63-68). (Communications in Computer and Information Science; 卷 57 CCIS). https://doi.org/10.1007/978-3-642-10616-3_9

Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. / Chen, Chien Ming; Uen, Yih Huei; Kuo, Chen Yi; Huang, Tzu Chuan; Lee, Jen Ai.

Communications in Computer and Information Science. 卷 57 CCIS 2009. p. 63-68 (Communications in Computer and Information Science; 卷 57 CCIS).

研究成果: 書貢獻/報告類型會議貢獻

Chen, CM, Uen, YH, Kuo, CY, Huang, TC & Lee, JA 2009, Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. 於 Communications in Computer and Information Science. 卷 57 CCIS, Communications in Computer and Information Science, 卷 57 CCIS, 頁 63-68, International Conference on Bio-Science and Bio-Technology, BSBT 2009. Held as Part of the Future Generation Information Technology Conference, FGIT 2009, Jeju Island, 大韓民國, 12/10/09. https://doi.org/10.1007/978-3-642-10616-3_9
Chen CM, Uen YH, Kuo CY, Huang TC, Lee JA. Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. 於 Communications in Computer and Information Science. 卷 57 CCIS. 2009. p. 63-68. (Communications in Computer and Information Science). https://doi.org/10.1007/978-3-642-10616-3_9
Chen, Chien Ming ; Uen, Yih Huei ; Kuo, Chen Yi ; Huang, Tzu Chuan ; Lee, Jen Ai. / Fluorimetric determination of L-3-hydroxybutyrate concentrations in the serum of normal and aristolochic acid-treated mice. Communications in Computer and Information Science. 卷 57 CCIS 2009. 頁 63-68 (Communications in Computer and Information Science).
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AB - We determined the concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of normal and aristolochic acid (AA)-treated mice. The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically by column-switching high-performance liquid chromatography (HPLC). Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for enantiomeric separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

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