Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs

Long Jyun Su, Meng Shiue Wu, Yuen Yung Hui, Be Ming Chang, Lei Pan, Pei Chen Hsu, Yit Tsong Chen, Hong Nerng Ho, Yen Hua Huang, Thai Yen Ling, Hsao Hsun Hsu, Huan Cheng Chang

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.
原文英語
文章編號45607
期刊Scientific Reports
7
DOIs
出版狀態已發佈 - 三月 30 2017

指紋

Nanodiamonds
Mesenchymal Stromal Cells
Swine
Placenta
Stem Cells
Animal Models
Membranes
Optical Imaging
Therapeutic Uses
Cell- and Tissue-Based Therapy
Intravenous Administration
Albumins
Rodentia
Pharmacokinetics
Therapeutics

ASJC Scopus subject areas

  • General

引用此文

Su, L. J., Wu, M. S., Hui, Y. Y., Chang, B. M., Pan, L., Hsu, P. C., ... Chang, H. C. (2017). Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs. Scientific Reports, 7, [45607]. https://doi.org/10.1038/srep45607

Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs. / Su, Long Jyun; Wu, Meng Shiue; Hui, Yuen Yung; Chang, Be Ming; Pan, Lei; Hsu, Pei Chen; Chen, Yit Tsong; Ho, Hong Nerng; Huang, Yen Hua; Ling, Thai Yen; Hsu, Hsao Hsun; Chang, Huan Cheng.

於: Scientific Reports, 卷 7, 45607, 30.03.2017.

研究成果: 雜誌貢獻文章

Su, LJ, Wu, MS, Hui, YY, Chang, BM, Pan, L, Hsu, PC, Chen, YT, Ho, HN, Huang, YH, Ling, TY, Hsu, HH & Chang, HC 2017, 'Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs', Scientific Reports, 卷 7, 45607. https://doi.org/10.1038/srep45607
Su, Long Jyun ; Wu, Meng Shiue ; Hui, Yuen Yung ; Chang, Be Ming ; Pan, Lei ; Hsu, Pei Chen ; Chen, Yit Tsong ; Ho, Hong Nerng ; Huang, Yen Hua ; Ling, Thai Yen ; Hsu, Hsao Hsun ; Chang, Huan Cheng. / Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs. 於: Scientific Reports. 2017 ; 卷 7.
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