Fluorescence in situ hybridization (FISH) as a method to detect aneuploid cells

Hsiao Jui Wei, Tsung Hsien Su, Chung Liang Chien, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Objectives: To test the sensitivity and specificity of various FISH probes for detecting male and aneuploid cells and to determine the percentage of fetal cells that must be present in a sample in order to use the probes for prenatal diagnosis. Methods: Adult human lymphocytes were cultured and harvested. Twelve different proportions of male to female cells and 5 different proportions of trisomy 21 cells and trisomy 18 cells in euploid cells were prepared for FISH. Alpha-satellite DYZ1 was applied to detect the male cells. Chromosome 21/Down syndrome critical region cosmid and D13Z1/D21Z1 alpha-satellite probes were applied to detect trisomy 21 cells. The D18Z1 alpha-satellite probe was used to detect trisomy 18 cells. Results: DYZ1 detected male cells reliably in concentrations as low as 2%. Both D18Z1 and chromosome 21/Down syndrome critical region cosmid probes could detect aneuploid cells at reasonably achievable concentrations. However, the D13Z1/D21Z1 probe was not sensitive below concentrations of 50%. Conclusions: FISH is an alternative technique for noninvasive prenatal diagnosis. The sensitivity and specificity of FISH probes may play a crucial role in the accuracy of prenatal diagnosis.

原文英語
頁(從 - 到)309-313
頁數5
期刊Fetal Diagnosis and Therapy
12
發行號5
出版狀態已發佈 - 1997

指紋

Aneuploidy
Fluorescence In Situ Hybridization
Prenatal Diagnosis
Chromosomes, Human, Pair 21
Cosmids
Down Syndrome
Sensitivity and Specificity
Lymphocytes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Pediatrics, Perinatology, and Child Health

引用此文

Fluorescence in situ hybridization (FISH) as a method to detect aneuploid cells. / Wei, Hsiao Jui; Su, Tsung Hsien; Chien, Chung Liang; Tzeng, Chii Ruey.

於: Fetal Diagnosis and Therapy, 卷 12, 編號 5, 1997, p. 309-313.

研究成果: 雜誌貢獻文章

Wei, Hsiao Jui ; Su, Tsung Hsien ; Chien, Chung Liang ; Tzeng, Chii Ruey. / Fluorescence in situ hybridization (FISH) as a method to detect aneuploid cells. 於: Fetal Diagnosis and Therapy. 1997 ; 卷 12, 編號 5. 頁 309-313.
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abstract = "Objectives: To test the sensitivity and specificity of various FISH probes for detecting male and aneuploid cells and to determine the percentage of fetal cells that must be present in a sample in order to use the probes for prenatal diagnosis. Methods: Adult human lymphocytes were cultured and harvested. Twelve different proportions of male to female cells and 5 different proportions of trisomy 21 cells and trisomy 18 cells in euploid cells were prepared for FISH. Alpha-satellite DYZ1 was applied to detect the male cells. Chromosome 21/Down syndrome critical region cosmid and D13Z1/D21Z1 alpha-satellite probes were applied to detect trisomy 21 cells. The D18Z1 alpha-satellite probe was used to detect trisomy 18 cells. Results: DYZ1 detected male cells reliably in concentrations as low as 2{\%}. Both D18Z1 and chromosome 21/Down syndrome critical region cosmid probes could detect aneuploid cells at reasonably achievable concentrations. However, the D13Z1/D21Z1 probe was not sensitive below concentrations of 50{\%}. Conclusions: FISH is an alternative technique for noninvasive prenatal diagnosis. The sensitivity and specificity of FISH probes may play a crucial role in the accuracy of prenatal diagnosis.",
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AU - Chien, Chung Liang

AU - Tzeng, Chii Ruey

PY - 1997

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N2 - Objectives: To test the sensitivity and specificity of various FISH probes for detecting male and aneuploid cells and to determine the percentage of fetal cells that must be present in a sample in order to use the probes for prenatal diagnosis. Methods: Adult human lymphocytes were cultured and harvested. Twelve different proportions of male to female cells and 5 different proportions of trisomy 21 cells and trisomy 18 cells in euploid cells were prepared for FISH. Alpha-satellite DYZ1 was applied to detect the male cells. Chromosome 21/Down syndrome critical region cosmid and D13Z1/D21Z1 alpha-satellite probes were applied to detect trisomy 21 cells. The D18Z1 alpha-satellite probe was used to detect trisomy 18 cells. Results: DYZ1 detected male cells reliably in concentrations as low as 2%. Both D18Z1 and chromosome 21/Down syndrome critical region cosmid probes could detect aneuploid cells at reasonably achievable concentrations. However, the D13Z1/D21Z1 probe was not sensitive below concentrations of 50%. Conclusions: FISH is an alternative technique for noninvasive prenatal diagnosis. The sensitivity and specificity of FISH probes may play a crucial role in the accuracy of prenatal diagnosis.

AB - Objectives: To test the sensitivity and specificity of various FISH probes for detecting male and aneuploid cells and to determine the percentage of fetal cells that must be present in a sample in order to use the probes for prenatal diagnosis. Methods: Adult human lymphocytes were cultured and harvested. Twelve different proportions of male to female cells and 5 different proportions of trisomy 21 cells and trisomy 18 cells in euploid cells were prepared for FISH. Alpha-satellite DYZ1 was applied to detect the male cells. Chromosome 21/Down syndrome critical region cosmid and D13Z1/D21Z1 alpha-satellite probes were applied to detect trisomy 21 cells. The D18Z1 alpha-satellite probe was used to detect trisomy 18 cells. Results: DYZ1 detected male cells reliably in concentrations as low as 2%. Both D18Z1 and chromosome 21/Down syndrome critical region cosmid probes could detect aneuploid cells at reasonably achievable concentrations. However, the D13Z1/D21Z1 probe was not sensitive below concentrations of 50%. Conclusions: FISH is an alternative technique for noninvasive prenatal diagnosis. The sensitivity and specificity of FISH probes may play a crucial role in the accuracy of prenatal diagnosis.

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KW - Flow cytometry

KW - Prenatal diagnosis

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