Flow cytometric DNA and cytomorphometric analysis in renal cell carcinoma: Its correlation with histopathology and prognosis

D. S. Yu, C. M. Hsu, W. H. Lee, S. Y. Chang, C. P. Ma

研究成果: 雜誌貢獻文章

13 引文 斯高帕斯(Scopus)

摘要

Cell material from 49 cases of archival paraffin-embedded tumor specimens of newly diagnosed renal cell carcinoma (RCC) was studied retrospectively using rapid flow cytofluorometric (propidium iodide) DNA analysis. The degree of ploidy (DNA index), percentage of cells in the S-phase (SPF), and modal nuclear size were determined from histograms. The tumors were classified as diploid (DNA index = 0.9-1.1) or aneuploid. Proliferative activities of the tumors were assessed from the proportion of S-phase cells. The aneuploid occurrence was 77.6% in our series. Univariate and multivariate logistic regression analyses of DNA ploidy and associated parameters showed that DNA ploidy was correlated with SPF of cells (P = 0.0061) as no correlation was seen between DNA ploidy, sex, age, histological type, tumor size, stage, and nuclear size (P = 0.0697). Multiple aneuploid stem lines had no influences on ploidy and prognosis. Comparison of survival data using the multivariate stepwise hazard rate and Lee-Desu statistics showed that patient prognosis was closely related to tumor size (P = 0.006) and staging (P <0.0001). DNA ploidy had marginal correlation to progression and disease-specific death (P = 0.064). Nevertheless, flow cytometric analysis in conjunction with conventional histopathology may have a potential role for the management of patients with RCC.

原文英語
頁(從 - 到)480-485
頁數6
期刊Journal of Surgical Research
55
發行號5
DOIs
出版狀態已發佈 - 1993
對外發佈Yes

ASJC Scopus subject areas

  • Surgery

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