Fiber type and myosin heavy chain compositions of adult pretarsal orbicularis oculi muscle

Nai Chen Cheng, Shu Lang Liao, I. Jong Wang, I. Chan Lin, Yueh B. Tang

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

Pretarsal orbicularis oculi muscle (POOM) is an important structure of eyelid movement in human. The aim of this study was to investigate fiber histomorphology and myosin heavy chain (MyHC) isoform composition of adult POOM, and to clarify their age-related changes. Eyelid specimens from 58 subjects (age range, 21 to 91 years) were collected during upper blepharoplasty procedures. Serial cross sections of POOM were ATPase-stained and examined under miscroscope. Quantitative measures of muscle fiber size and fiber type distribution were obtained in 35 subjects with adequate fiber cross sections. Relative MyHC isoform contents of POOM were retrieved by gel electrophoresis in all 58 subjects. Examination of the histochemical staining revealed an abundance of type II fiber ( >85%) in human POOM, with more type IIX than IIA fibers. Decreased mean area of all fibers and type IIA fibers were noted in the old group when compared to the young. As for MyHC analysis, the relative content of MyHC isoforms exhibited an order of IIX > IIA > I, and the relative MyHC IIA content showed a negative correlation with age. Comparing with previous studies of limb or masticatory muscles, adult POOM exhibits a unique fiber and MyHC composition, as well as a different aging pattern.

原文英語
頁(從 - 到)177-182
頁數6
期刊Journal of Molecular Histology
38
發行號3
DOIs
出版狀態已發佈 - 六月 2007
對外發佈Yes

指紋

Myosin Heavy Chains
Muscles
Protein Isoforms
Eyelids
Blepharoplasty
Masticatory Muscles
Adenosine Triphosphatases
Electrophoresis
Extremities
Gels
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

引用此文

Fiber type and myosin heavy chain compositions of adult pretarsal orbicularis oculi muscle. / Cheng, Nai Chen; Liao, Shu Lang; Wang, I. Jong; Lin, I. Chan; Tang, Yueh B.

於: Journal of Molecular Histology, 卷 38, 編號 3, 06.2007, p. 177-182.

研究成果: 雜誌貢獻文章

Cheng, Nai Chen ; Liao, Shu Lang ; Wang, I. Jong ; Lin, I. Chan ; Tang, Yueh B. / Fiber type and myosin heavy chain compositions of adult pretarsal orbicularis oculi muscle. 於: Journal of Molecular Histology. 2007 ; 卷 38, 編號 3. 頁 177-182.
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abstract = "Pretarsal orbicularis oculi muscle (POOM) is an important structure of eyelid movement in human. The aim of this study was to investigate fiber histomorphology and myosin heavy chain (MyHC) isoform composition of adult POOM, and to clarify their age-related changes. Eyelid specimens from 58 subjects (age range, 21 to 91 years) were collected during upper blepharoplasty procedures. Serial cross sections of POOM were ATPase-stained and examined under miscroscope. Quantitative measures of muscle fiber size and fiber type distribution were obtained in 35 subjects with adequate fiber cross sections. Relative MyHC isoform contents of POOM were retrieved by gel electrophoresis in all 58 subjects. Examination of the histochemical staining revealed an abundance of type II fiber ( >85{\%}) in human POOM, with more type IIX than IIA fibers. Decreased mean area of all fibers and type IIA fibers were noted in the old group when compared to the young. As for MyHC analysis, the relative content of MyHC isoforms exhibited an order of IIX > IIA > I, and the relative MyHC IIA content showed a negative correlation with age. Comparing with previous studies of limb or masticatory muscles, adult POOM exhibits a unique fiber and MyHC composition, as well as a different aging pattern.",
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