Fatty acids modulate transforming growth factor-beta activity and plasma clearance.

Thai Yen Ling, Yen Hua Huang, Ming Chih Lai, Shuan Shian Huang, Jung San Huang

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

The activity and plasma clearance of transforming growth factor (TGF)-beta are known to be regulated by activated alpha2-macroglobulin (alpha2M*). This has been implicated in pathophysiological processes, but no small molecule compounds have been reported to modulate TGF-beta activity by affecting the interaction of TGF-beta and alpha2M*. Here, we demonstrate that fatty acids are capable of inhibiting complex formation of TGF-beta isoforms and alpha2M* as demonstrated by nondenaturing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is dependent on carbon chain length (C20, C18, C16, C14 > C12 > C10), degree of unsaturation (polyunsaturated > saturated), and TGF-beta isoforms (TGF-beta1 > TGF-beta2 > TGF-beta3). Arachidonic acid, which is one of the most potent inhibitors, is also capable of dissociating TGF-beta-alpha2M* complexes, but higher concentrations are required. Arachidonic acid appears to inhibit TGF-beta-alpha2M* complex formation by binding specifically to alpha2M* as demonstrated by gel filtration chromatography. Arachidonic acid reverses the inhibitory effect of alpha2M* on TGF-beta binding, TGF-beta-induced growth inhibition, and TGF-beta-induced transcriptional activation in mink lung epithelial cells and affects plasma clearance of TGF-beta-alpha2M* complexes in mice. These results show that fatty acids are effective modulators of TGF-beta activity and plasma clearance and may be useful in treating human diseases through their effects on the interaction of TGF-beta and alpha2M*.

原文英語
頁(從 - 到)1559-1561
頁數3
期刊FASEB Journal
17
發行號11
出版狀態已發佈 - 2003
對外發佈Yes

指紋

Transforming Growth Factor beta
alpha-Macroglobulins
Fatty Acids
Thermodynamic properties
Plasmas
Arachidonic Acid
Protein Isoforms
Transforming Growth Factor beta3
Transforming Growth Factor beta2
Mink
Transforming Growth Factor beta1
Chromatography
Electrophoresis
Chain length
Sodium Dodecyl Sulfate
Modulators
Transcriptional Activation
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Carbon

引用此文

Ling, T. Y., Huang, Y. H., Lai, M. C., Huang, S. S., & Huang, J. S. (2003). Fatty acids modulate transforming growth factor-beta activity and plasma clearance. FASEB Journal, 17(11), 1559-1561.

Fatty acids modulate transforming growth factor-beta activity and plasma clearance. / Ling, Thai Yen; Huang, Yen Hua; Lai, Ming Chih; Huang, Shuan Shian; Huang, Jung San.

於: FASEB Journal, 卷 17, 編號 11, 2003, p. 1559-1561.

研究成果: 雜誌貢獻文章

Ling, TY, Huang, YH, Lai, MC, Huang, SS & Huang, JS 2003, 'Fatty acids modulate transforming growth factor-beta activity and plasma clearance.', FASEB Journal, 卷 17, 編號 11, 頁 1559-1561.
Ling, Thai Yen ; Huang, Yen Hua ; Lai, Ming Chih ; Huang, Shuan Shian ; Huang, Jung San. / Fatty acids modulate transforming growth factor-beta activity and plasma clearance. 於: FASEB Journal. 2003 ; 卷 17, 編號 11. 頁 1559-1561.
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