Facilitated and controlled strontium ranelate delivery using gcs-ha nanocarriers embedded into pegda coupled with decortication driven spinal regeneration

Chih Wei Chiang, Chih Hwa Chen, Yankuba B. Manga, Shao Chan Huang, Kun Mao Chao, Pei Ru Jheng, Pei Chun Wong, Batzaya Nyambat, Mantosh Kumar Satapathy, Er Yuan Chuang

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Background and Purpose: Strontium ranelate (SrR) is an oral pharmaceutical agent for osteoporosis. In recent years, numerous unwanted side effects of oral SrR have been revealed. Therefore, its clinical administration and applications are limited. Hereby, this study aims to develop, formulate, and characterize an effective SrR carrier system for spinal bone regeneration. Methods: Herein, glycol chitosan with hyaluronic acid (HA)-based nanoformulation was used to encapsulate SrR nanoparticles (SrRNPs) through electrostatic interaction. Afterward, the poly(ethylene glycol) diacrylate (PEGDA)-based hydrogels were used to encapsulate pre-synthesized SrRNPs (SrRNPs-H). The scanning electron microscope (SEM), TEM, rhe-ometer, Fourier-transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS) were used to characterize prepared formulations. The rabbit osteoblast and a rat spinal decortication models were used to evaluate and assess the developed formulation biocompat-ibility and therapeutic efficacy. Results: In vitro and in vivo studies for cytotoxicity and bone regeneration were conducted. The cell viability test showed that SrRNPs exerted no cytotoxic effects in osteoblast in vitro. Furthermore, in vivo analysis for new bone regeneration mechanism was carried out on rat decortication models. Radiographical and histological analysis suggested a higher level of bone regeneration in the SrRNPs-H-implanted groups than in the other experimental groups. Conclusion: Local administration of the newly developed formulated SrR could be a promising alternative therapy to enhance bone regeneration in bone-defect sites in future clinical applications.

原文英語
頁(從 - 到)4209-4224
頁數16
期刊International Journal of Nanomedicine
16
DOIs
出版狀態已發佈 - 2021

ASJC Scopus subject areas

  • 生物物理學
  • 生物工程
  • 生物材料
  • 藥學科學
  • 藥物發現
  • 有機化學

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