Facile method for determination of deoxycytidine kinase activity in biological milieus

Wei Hua Hao, Li Chieh Yang, Jong Jing Wang, Chang Shan Hsu, Li Chien Chang, Kuang Yang Hsu

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)

摘要

A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.

原文英語
頁(從 - 到)236-241
頁數6
期刊Journal of Food and Drug Analysis
22
發行號2
DOIs
出版狀態已發佈 - 6月 1 2014

ASJC Scopus subject areas

  • 食品科學
  • 藥理

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