Fabrication of Polylactic Acid/β-Tricalcium Phosphate FDM 3D Printing Fiber to Enhance Osteoblastic-Like Cell Performance

Eisner Salamanca, Ting Chia Tsao, Hao Wen Hseuh, Yi Fan Wu, Cheuk Sing Choy, Chin Kai Lin, Yu Hwa Pan, Nai Chia Teng, Mao Chuan Huang, Shang Ming Lin, Wei Jen Chang

研究成果: 雜誌貢獻文章同行評審

摘要

Guided-bone regeneration (GBR) is increasingly using three-dimensional (3D) printing by fused deposition modeling (FDM) to build the filaments used for treatment. Polylactic acid (PLA) and beta-tricalcium phosphate (β-TCP) are widely used as base materials in 3D printing, necessitating that they are studied together in the context of GBR treatment. This study sought to test and compare the properties and efficacy of different ratios of β-TCP and PLA used to make the 3D-printed filament material to find the most effective combination of materials for GBR treatment. Several ratios of PLA to β-TCP were tested, including PLA/β-TCP ratios of 95%/5% (PLA-5), 90%/10% (PLA-10), 85%/15% (PLA-15), and 80%/20% (PLA-20), and quantitative real-time polymerase chain reaction (qPCR) in vitro testing was done to characterize the material. After adding β-TCP to PLA, mechanical testing indicated that tensile and elongation strengths decreased, hardness was retained, and cell proliferation was promoted. The effect of PLA and β-TCP on increasing alkaline phosphatase (ALP) activity was significantly greater in a ratio of 10% β-TCP/90% PLA at 5 days (p < 0.05) than in any other ratios tested. This is supported by results from qPCR testing, which showed early osteoblast-like differentiation of DLX5, RUNX2, OPG, OC, and collagen type 1 (COL-1) expression levels similar to cells cultured on PLA-10. Our results demonstrated that 3D printing of filaments produced in a ratio of 90% PLA to 10% β-TCP was more effective for GBR than that of filaments produced only using PLA.

原文英語
文章編號683706
期刊Frontiers in Materials
8
DOIs
出版狀態已發佈 - 五月 28 2021

ASJC Scopus subject areas

  • 材料科學(雜項)

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