Fabrication of implantable, enzyme-immobilized glutamate sensors for the monitoring of glutamate concentration changes in vitro and in vivo

Tina T C Tseng, Cheng Fu Chang, Wen Chin Chan

研究成果: 雜誌貢獻文章

21 引文 斯高帕斯(Scopus)

摘要

Glutamate sensors based on the immobilization of glutamate oxidase (GlutOx) were prepared by adsorption on electrodeposited chitosan (Method 1) and by crosslinking with glutaraldehyde (Method 2) on micromachined platinum microelectrodes. It was observed that glutamate sensors prepared by Method 1 have faster response time (-1•cm-2, N = 12) compared to those prepared by Method 1 (linear detection range: 20-217 μM and sensitivity: 34.9 ± 4.8 nA•μM-1•cm-2, N = 8). The applicability of the glutamate sensors in vivo was also demonstrated. The glutamate sensors were implanted into the rat brain to monitor the stress-induced extracellular glutamate release in the hypothalamus of the awake, freely moving rat.

原文英語
頁(從 - 到)7341-7355
頁數15
期刊Molecules
19
發行號6
DOIs
出版狀態已發佈 - 2014

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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