Fabrication of biomolecules coated nanostructured oxide layer to facilitate cell adhesion and proliferation for improving osseointegration

Ling Chuan Hsu, Wen Chien Lan, Keng Liang Ou, Hsin Hua Chou, Shih Cheng Wen, Chung Ming Liu, Kazuhiko Endo, Mao Suan Huang, Chiung Fang Huang

研究成果: 雜誌貢獻文章

摘要

This study was to elucidate the surface characteristics and in vitro cell behaviors of biomedical titanium (Ti) with the biofunctional surface (nanostructured rutile-Ti dioxide (NanoR-TiO2) layer combined with amino-groups (NH2) and albumin). Biofunctional surface features were analyzed via scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Immunolabeling with nano-gold particles was used to reflect the expected specific binding of protein on the biofunctional surface. In vitro cell behaviors were evaluated by culturing with mouse pre-osteoblastic cell line (MC3T3-E1) at 37 °C in different periods. Analytical results indicated that the sandwich-like (NH2/NanoR-TiO2/Ti) layer was formed on the Ti surface after plasma oxidation and polymerization. Immunolabeling analysis results also proved that the albumin can be successfully bonded with NH2 on NanoR-TiO2 layer. Moreover, cell morphology observation revealed that the biofunctional surface could potentially facilitate cell adhesion and proliferation. Therefore, the biomedical Ti with the biofunctional surface is a promising biomaterial for dental implant applications.

原文英語
期刊Ceramics International
DOIs
出版狀態已發佈 - 一月 1 2019

指紋

Cell adhesion
Cell proliferation
Biomolecules
Oxides
Fabrication
Titanium
Albumins
Dental prostheses
Biocompatible Materials
Biomaterials
Gold
Titanium dioxide
Atomic force microscopy
Carrier Proteins
Polymerization
Cells
Transmission electron microscopy
Proteins
Plasmas
Oxidation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry

引用此文

Fabrication of biomolecules coated nanostructured oxide layer to facilitate cell adhesion and proliferation for improving osseointegration. / Hsu, Ling Chuan; Lan, Wen Chien; Ou, Keng Liang; Chou, Hsin Hua; Wen, Shih Cheng; Liu, Chung Ming; Endo, Kazuhiko; Huang, Mao Suan; Huang, Chiung Fang.

於: Ceramics International, 01.01.2019.

研究成果: 雜誌貢獻文章

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abstract = "This study was to elucidate the surface characteristics and in vitro cell behaviors of biomedical titanium (Ti) with the biofunctional surface (nanostructured rutile-Ti dioxide (NanoR-TiO2) layer combined with amino-groups (NH2) and albumin). Biofunctional surface features were analyzed via scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Immunolabeling with nano-gold particles was used to reflect the expected specific binding of protein on the biofunctional surface. In vitro cell behaviors were evaluated by culturing with mouse pre-osteoblastic cell line (MC3T3-E1) at 37 °C in different periods. Analytical results indicated that the sandwich-like (NH2/NanoR-TiO2/Ti) layer was formed on the Ti surface after plasma oxidation and polymerization. Immunolabeling analysis results also proved that the albumin can be successfully bonded with NH2 on NanoR-TiO2 layer. Moreover, cell morphology observation revealed that the biofunctional surface could potentially facilitate cell adhesion and proliferation. Therefore, the biomedical Ti with the biofunctional surface is a promising biomaterial for dental implant applications.",
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author = "Hsu, {Ling Chuan} and Lan, {Wen Chien} and Ou, {Keng Liang} and Chou, {Hsin Hua} and Wen, {Shih Cheng} and Liu, {Chung Ming} and Kazuhiko Endo and Huang, {Mao Suan} and Huang, {Chiung Fang}",
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AU - Hsu, Ling Chuan

AU - Lan, Wen Chien

AU - Ou, Keng Liang

AU - Chou, Hsin Hua

AU - Wen, Shih Cheng

AU - Liu, Chung Ming

AU - Endo, Kazuhiko

AU - Huang, Mao Suan

AU - Huang, Chiung Fang

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N2 - This study was to elucidate the surface characteristics and in vitro cell behaviors of biomedical titanium (Ti) with the biofunctional surface (nanostructured rutile-Ti dioxide (NanoR-TiO2) layer combined with amino-groups (NH2) and albumin). Biofunctional surface features were analyzed via scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Immunolabeling with nano-gold particles was used to reflect the expected specific binding of protein on the biofunctional surface. In vitro cell behaviors were evaluated by culturing with mouse pre-osteoblastic cell line (MC3T3-E1) at 37 °C in different periods. Analytical results indicated that the sandwich-like (NH2/NanoR-TiO2/Ti) layer was formed on the Ti surface after plasma oxidation and polymerization. Immunolabeling analysis results also proved that the albumin can be successfully bonded with NH2 on NanoR-TiO2 layer. Moreover, cell morphology observation revealed that the biofunctional surface could potentially facilitate cell adhesion and proliferation. Therefore, the biomedical Ti with the biofunctional surface is a promising biomaterial for dental implant applications.

AB - This study was to elucidate the surface characteristics and in vitro cell behaviors of biomedical titanium (Ti) with the biofunctional surface (nanostructured rutile-Ti dioxide (NanoR-TiO2) layer combined with amino-groups (NH2) and albumin). Biofunctional surface features were analyzed via scanning electron microscopy, atomic force microscopy, and transmission electron microscopy. Immunolabeling with nano-gold particles was used to reflect the expected specific binding of protein on the biofunctional surface. In vitro cell behaviors were evaluated by culturing with mouse pre-osteoblastic cell line (MC3T3-E1) at 37 °C in different periods. Analytical results indicated that the sandwich-like (NH2/NanoR-TiO2/Ti) layer was formed on the Ti surface after plasma oxidation and polymerization. Immunolabeling analysis results also proved that the albumin can be successfully bonded with NH2 on NanoR-TiO2 layer. Moreover, cell morphology observation revealed that the biofunctional surface could potentially facilitate cell adhesion and proliferation. Therefore, the biomedical Ti with the biofunctional surface is a promising biomaterial for dental implant applications.

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