Fabrication of a bioengineered corneal endothelial cell sheet using chitosan/polycaprolactone blend membranes

Tai Horng Young, I. Jong Wang, Fung Rong Hu, Tsung Jen Wang

研究成果: 雜誌貢獻文章同行評審

36 引文 斯高帕斯(Scopus)

摘要

Our previous study has demonstrated cultivation of bovine corneal endothelial cells (BCECs) on the chitosan-polycaprolactone (PCL) blends. BCECs could grow well and express physiological phenotypes after PCL was introduced into chitosan by means of blending. The feasibility of using chitosan-PCL-blended membranes as scaffold and carrier for corneal endothelial cell (CEC) transplantation is worthy of more investigation. In this work, we attempt to manufacture various blended membranes to bring BCEC cultivation into harmony by hybridizing two polymers with fine adjustment. Therefore, chitosan, which does not promote BCECs maturation, and PCL, which supports, BCEC proliferation, are combined to prepare the blends. Analyses of transmittance, proliferative, abilities, phenotypic expressions, extracellular matrix (ECM) protein production, and hemotoxylin and eosin (H&E) staining were executed step-by-step. From our observations, the blended membranes united respective characteristics. The cultured BCECs on the blends illustrated normal appearance and good proliferative abilities. Immunohistochemical staining verified physiologically phenotypic expressions of ZO-1 and Na-K ATPase. Western blot analysis also confirmed the existence of collagen type IV proteins. Furthermore, the formation of a continuous monolayer of cells attached on the blended membrane was confirmed by H&E staining. These results suggested that chitosan-PCL blended membranes may be an optimized biomaterial to fabricate bioengineered corneal endothelium and show potential to facilitate CEC transplantation in the future.

原文英語
頁(從 - 到)403-410
頁數8
期刊Colloids and Surfaces B: Biointerfaces
116
DOIs
出版狀態已發佈 - 4月 1 2014

ASJC Scopus subject areas

  • 生物技術
  • 膠體和表面化學
  • 物理與理論化學
  • 表面和介面
  • 醫藥 (全部)

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