Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis

Yen Hsiang Chang, Zhi Meng Chen, Hsien Yu Peng, Yuan Jen Tsai, Tzer Bin Lin, Gin Den Chen, C. R. Chris Wang, Sarah Y. Chang

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.

原文英語
頁(從 - 到)457-462
頁數6
期刊Journal of the Chinese Chemical Society
58
發行號4
DOIs
出版狀態已發佈 - 2011
對外發佈Yes

指紋

Substance P
Desorption
Nanoparticles
Lasers
Ionization
Rats
Coulomb interactions
ferric oxide
Washing
Surface-Active Agents
Buffers
Salts
Adsorption
Recovery

ASJC Scopus subject areas

  • Chemistry(all)

引用此文

Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis. / Chang, Yen Hsiang; Chen, Zhi Meng; Peng, Hsien Yu; Tsai, Yuan Jen; Lin, Tzer Bin; Chen, Gin Den; Chris Wang, C. R.; Chang, Sarah Y.

於: Journal of the Chinese Chemical Society, 卷 58, 編號 4, 2011, p. 457-462.

研究成果: 雜誌貢獻文章

Chang, Yen Hsiang ; Chen, Zhi Meng ; Peng, Hsien Yu ; Tsai, Yuan Jen ; Lin, Tzer Bin ; Chen, Gin Den ; Chris Wang, C. R. ; Chang, Sarah Y. / Extraction of substance P by functionalized iron oxide nanoparticles for matrix-assisted laser desorption/lonization mass spectrometric analysis. 於: Journal of the Chinese Chemical Society. 2011 ; 卷 58, 編號 4. 頁 457-462.
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abstract = "Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7{\%}, respectively.",
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AU - Chang, Yen Hsiang

AU - Chen, Zhi Meng

AU - Peng, Hsien Yu

AU - Tsai, Yuan Jen

AU - Lin, Tzer Bin

AU - Chen, Gin Den

AU - Chris Wang, C. R.

AU - Chang, Sarah Y.

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AB - Functionalized iron oxide nanoparticles (IONPs) were used as concentrating probes to extract substance P (SP) from aqueous solution before matrix-assisted laser desorption/ionization mass spectrometric (MALDI/MS) analysis. Adsorption of SP onto the nanoparticles was mainly due to electrostatic interaction. After simple washing, the SP-adsorbed nanoparticles were directly analyzed by MALDI/MS. With this concentration strategy, the limit of detection (LOD) of SP in 500 μL buffer solution (pH 8) was 0.1 nM. In addition, the nanoparticles could isolate SP from a solution with high contents of salts and surfactants, thus alleviating suppression effect during MALDI/MS analysis. This new method was successfully applied to the determination of SP in rat spinal cord samples. The LOD and recovery of SP in a rat spinal cord sample were 3.1 nM and 85.7%, respectively.

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