External quality assessment of sputum smear microscopy in taiwan

M. H. Wu, C. Y. Chiang, R. Jou, S. Y. Chang, K. T. Luh

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

OBJECTIVE: To evaluate the quality of sputum smear microscopy in nine Taiwan Centers for Disease Control contract laboratories, an external quality assessment (EQA) programme has been implemented since 2005. DESIGN: A sampling strategy based on the lot quality assurance system was applied to select slides for rechecking. Supervisory visits and technical training were conducted to determine the causes of errors and to take corrective action. RESULTS: Of the 1017 slides sampled in 2005, 637 (63%) had proper smear size, 492 (48%) proper thickness and 884 (87%) proper staining; the corresponding figures were 972 (100%), 748 (77%) and 809 (99.6%) for the 972 slides rechecked in 2006. After training, the quality of size and staining of smear preparation had significantly improved (P <0.001) in 2006. Rechecking of 981 readable slides in 2005 identified 3 (0.3%) high false-negatives, 3 (16.7%) low false-positives and 26 (2.8%) low false-negatives; the corresponding errors were 3 (0.3%), 8 (28.6%) and 12 (1.3%) for the 972 slides rechecked in 2006. Of the eight laboratories, two (25%) and four (50%) reached 80% sensitivity in 2005 and 2006, respectively. CONCLUSION: Technical training and EQA improved the quality of sputum smear microscopy services.

原文英語
頁(從 - 到)606-612
頁數7
期刊International Journal of Tuberculosis and Lung Disease
13
發行號5
出版狀態已發佈 - 2009
對外發佈Yes

指紋

Sputum
Taiwan
Microscopy
Staining and Labeling
Centers for Disease Control and Prevention (U.S.)
Contracts

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

引用此文

External quality assessment of sputum smear microscopy in taiwan. / Wu, M. H.; Chiang, C. Y.; Jou, R.; Chang, S. Y.; Luh, K. T.

於: International Journal of Tuberculosis and Lung Disease, 卷 13, 編號 5, 2009, p. 606-612.

研究成果: 雜誌貢獻文章

Wu, M. H. ; Chiang, C. Y. ; Jou, R. ; Chang, S. Y. ; Luh, K. T. / External quality assessment of sputum smear microscopy in taiwan. 於: International Journal of Tuberculosis and Lung Disease. 2009 ; 卷 13, 編號 5. 頁 606-612.
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abstract = "OBJECTIVE: To evaluate the quality of sputum smear microscopy in nine Taiwan Centers for Disease Control contract laboratories, an external quality assessment (EQA) programme has been implemented since 2005. DESIGN: A sampling strategy based on the lot quality assurance system was applied to select slides for rechecking. Supervisory visits and technical training were conducted to determine the causes of errors and to take corrective action. RESULTS: Of the 1017 slides sampled in 2005, 637 (63{\%}) had proper smear size, 492 (48{\%}) proper thickness and 884 (87{\%}) proper staining; the corresponding figures were 972 (100{\%}), 748 (77{\%}) and 809 (99.6{\%}) for the 972 slides rechecked in 2006. After training, the quality of size and staining of smear preparation had significantly improved (P <0.001) in 2006. Rechecking of 981 readable slides in 2005 identified 3 (0.3{\%}) high false-negatives, 3 (16.7{\%}) low false-positives and 26 (2.8{\%}) low false-negatives; the corresponding errors were 3 (0.3{\%}), 8 (28.6{\%}) and 12 (1.3{\%}) for the 972 slides rechecked in 2006. Of the eight laboratories, two (25{\%}) and four (50{\%}) reached 80{\%} sensitivity in 2005 and 2006, respectively. CONCLUSION: Technical training and EQA improved the quality of sputum smear microscopy services.",
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