Expression profile of tyrosine kinases in breast cancer

Funda Meric, Wei Ping Lee, Aysegul Sahin, Haixia Zhang, Hsing Jien Kung, Mien Chie Hung

研究成果: 雜誌貢獻文章同行評審

121 引文 斯高帕斯(Scopus)

摘要

The tyrosine kinase (TK) family includes many growth factor receptors, cell cycle regulators, and oncoproteins. Moreover, the receptor TKs HER2/neu and epidermal growth factor receptor are overexpressed in a subgroup of breast tumors and correlate with more aggressive behavior. Thus, TKs are being actively pursued as therapeutic targets. The purpose of this study was to determine the expression pattern of TKs in breast cancer. Reverse transcription-PCR was performed with degenerate primers based on conserved motifs of the catalytic domains of TKs, and the identities of the reverse transcription-PCR products were determined by digestion with a panel of restriction enzymes. Using a TK display assay, we studied the TK profiles of 13 breast cancer cell lines and two normal immortalized breast epithelial cell lines. The TK display assay reproducibly demonstrated known differences in HER-2/neu expression between cell lines. Several TKs, including receptor TKs Axl, Cak, fibroblast growth factor receptor 4, HEK8, HER2/neu, c-MET, RET, and nonreceptor TKs ARG, BRK, Janus kinase 1, Rak, and YES were detected in breast cancer cells. Several kinases were differentially expressed among the cell lines. Similar TK profiles were found using RNA from human breast tumors. We conclude that there is significant variability in the TK expression pattern of breast cancers. This variability should be considered when selecting TK inhibitors to treat patients.
原文英語
頁(從 - 到)361-367
頁數7
期刊Clinical Cancer Research
8
發行號2
出版狀態已發佈 - 1月 1 2002
對外發佈

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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