Expression of leukemia inhibitory factor and its receptor in preimplantation embryos

Hsin Fu Chen, Jin Yuh Shew, Hong Nerng Ho, Wei Li Hsu, Yu Shih Yang

研究成果: 雜誌貢獻文章

85 引文 (Scopus)

摘要

Objective: To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos. Design: Prospective study. Setting: University medical center. Patient(s): Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice. Intervention(s): Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study. Main Outcome Measure(s): The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology. Result(s): Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent. Conclusion(s): Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.
原文英語
頁(從 - 到)713-719
頁數7
期刊Fertility and Sterility
72
發行號4
DOIs
出版狀態已發佈 - 十月 1 1999
對外發佈Yes

指紋

OSM-LIF Receptors
Leukemia Inhibitory Factor
Blastocyst
Embryonic Structures
Oocytes
vpr Genes
Inbred ICR Mouse
Messenger RNA
Intracytoplasmic Sperm Injections
Reverse Transcription
Embryonic Development

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

引用此文

Expression of leukemia inhibitory factor and its receptor in preimplantation embryos. / Chen, Hsin Fu; Shew, Jin Yuh; Ho, Hong Nerng; Hsu, Wei Li; Yang, Yu Shih.

於: Fertility and Sterility, 卷 72, 編號 4, 01.10.1999, p. 713-719.

研究成果: 雜誌貢獻文章

Chen, Hsin Fu ; Shew, Jin Yuh ; Ho, Hong Nerng ; Hsu, Wei Li ; Yang, Yu Shih. / Expression of leukemia inhibitory factor and its receptor in preimplantation embryos. 於: Fertility and Sterility. 1999 ; 卷 72, 編號 4. 頁 713-719.
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abstract = "Objective: To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos. Design: Prospective study. Setting: University medical center. Patient(s): Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice. Intervention(s): Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study. Main Outcome Measure(s): The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology. Result(s): Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent. Conclusion(s): Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.",
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T1 - Expression of leukemia inhibitory factor and its receptor in preimplantation embryos

AU - Chen, Hsin Fu

AU - Shew, Jin Yuh

AU - Ho, Hong Nerng

AU - Hsu, Wei Li

AU - Yang, Yu Shih

PY - 1999/10/1

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N2 - Objective: To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos. Design: Prospective study. Setting: University medical center. Patient(s): Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice. Intervention(s): Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study. Main Outcome Measure(s): The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology. Result(s): Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent. Conclusion(s): Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.

AB - Objective: To examine the expression of leukemia inhibitory factor (LIF) and its receptor (LIF-R) transcripts in human and murine preimplantation embryos. Design: Prospective study. Setting: University medical center. Patient(s): Human oocytes were obtained from patients undergoing IVF treatment. Two-cell murine embryos were obtained from ICR strain mice. Intervention(s): Second-day intracytoplasmic sperm injection procedures were performed on oocytes that failed to be fertilized by IVF. Embryos were cultured to various stages and collected for study. Main Outcome Measure(s): The transcript levels of LIF and LIF-R in these embryos were examined and semiquantitated using single-cell reverse transcription-polymerase chain reaction methodology. Result(s): Leukemia inhibitory factor and LIF-R transcripts were detectable in most human preimplantation embryos (30 of 34 and 31 of 34 embryos showed LIF and LIF-R messenger RNA, respectively). There was a trend toward decreased expression of both transcripts in embryos at the four-cell stage and in embryos in which growth had been arrested for 24-48 hours. The expression of LIF and LIF-R genes in murine embryos was inconsistent. Conclusion(s): Preimplantation human embryos express LIF and LIF-R messenger RNA. It is suggested that LIF may be able to affect embryo development through its action at stages before implantation in an autocrine or paracrine manner.

KW - Cytokine

KW - Implantation

KW - Leukemia inhibitory factor

KW - Leukemia inhibitory factor receptor

KW - Preimplantation embryo

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