Expression of interleukin-17F in a mouse model of allergic asthma

Shintaro Suzuki, Fumio Kokubu, Mio Kawaguchi, Tetsuya Homma, Miho Odaka, Shin Watanabe, Koshi Ieki, Satoshi Matsukura, Masatsugu Kurokawa, Hiroko Takeuchi, Yoshiko Sasaki, Shau Ku Huang, Mitsuru Adachi, Hidekazu Ota

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46 引文 斯高帕斯(Scopus)


Background: Interleukin (IL)-17F is a recently discovered cytokine and is derived from a panel of limited cell types, such as activated CD4+ T cells, basophils, and mast cells. IL-17F is known to induce several cytokines and chemokines. However, its involvement in airway inflammation has not been well understood. To this end, the expression of IL-17F and the inhibitory effects of glucocorticoids on its expression in a mouse model of asthma were examined. Methods: Five-week-old BALB/c male mice were sensitized by intraperitoneal injection (i.p.) of ovalbumin (OVA) with alum, and challenged by daily inhalation of aerosolized 1% OVA. 24 h after last challenge (OVA/OVA), the expression of IL-17F was examined in lung tissues by immunohistochemistry and reverse-transcription polymerase chain reaction. Control mice were sensitized and challenged with saline (Sham/Sham). In addition, a group of OVA-sensitized mice received i.p. injection of water-soluble dexamethasone (DEX) in saline 1 h before OVA challenge (OVA/DEX). Results: In sham-challenged mice, IL-17F was not expressed in the lungs, while, in contrast, IL-17F was predominantly expressed in bronchial epithelial cells in addition to the infiltrating inflammatory cells in OVA/OVA mice. Further, the expression of IL-17 F was significantly attenuated by the treatment of mice with DEX. Conclusion: These results suggest that bronchial epithelium-derived IL-17F may represent a new pharmacological target for glucocorticoids and may play a role in allergic asthma.

頁(從 - 到)89-94
期刊International Archives of Allergy and Immunology
發行號SUPPL. 1
出版狀態已發佈 - 6月 1 2007

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學


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