Expression of heat shock cognate 70 in breast cancer

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Objectives: The aim of the present study was to obtain a general profile of heat shock cognate 70 gene (HSC70) expression in breast cancer. Materials and Methods: Five breast cancer cell lines plus one non-malignant breast epithelial cell line, and seven breast cancer tissues plus one normal breast tissue sample were selected for this study. RNA was extracted from these samples using standard procedures. The HSC70 expression was assessed by Northern blot analysis using a cDNA fragment of HSC70 as the probe. Two blots, one for cell line samples, the other for tissue samples, were performed. The signal density of each sample was calibrated against a respective loading control. Results: In the blot of cell line, the expression levels of HSC70 were similar among the breast cancer cell lines and the non-malignant cell line except for T47D, which displayed a 3.3-fold of the signal density than that of non-malignant cell line. In the blot of tissue, the variation of the HSC70 expression among the samples was narrow as well, however, one of the samples displayed a 2.5-fold greater amount of the signal density than that of the normal breast tissue counterpart. Conclusion: Most of the breast cancers and normal counterparts expressed similar levels of HSC70. However, a small fraction of the breast cancers over-expressed HSC70.
原文英語
頁(從 - 到)21-25
頁數5
期刊Tzu Chi Medical Journal
15
發行號1
出版狀態已發佈 - 二月 1 2003
對外發佈Yes

指紋

Shock
Hot Temperature
Breast Neoplasms
Cell Line
Gene Expression
Genes
Breast
Northern Blotting
Complementary DNA
Epithelial Cells
RNA

ASJC Scopus subject areas

  • Medicine(all)

引用此文

Expression of heat shock cognate 70 in breast cancer. / Kao, Ruey H.

於: Tzu Chi Medical Journal, 卷 15, 編號 1, 01.02.2003, p. 21-25.

研究成果: 雜誌貢獻文章

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AB - Objectives: The aim of the present study was to obtain a general profile of heat shock cognate 70 gene (HSC70) expression in breast cancer. Materials and Methods: Five breast cancer cell lines plus one non-malignant breast epithelial cell line, and seven breast cancer tissues plus one normal breast tissue sample were selected for this study. RNA was extracted from these samples using standard procedures. The HSC70 expression was assessed by Northern blot analysis using a cDNA fragment of HSC70 as the probe. Two blots, one for cell line samples, the other for tissue samples, were performed. The signal density of each sample was calibrated against a respective loading control. Results: In the blot of cell line, the expression levels of HSC70 were similar among the breast cancer cell lines and the non-malignant cell line except for T47D, which displayed a 3.3-fold of the signal density than that of non-malignant cell line. In the blot of tissue, the variation of the HSC70 expression among the samples was narrow as well, however, one of the samples displayed a 2.5-fold greater amount of the signal density than that of the normal breast tissue counterpart. Conclusion: Most of the breast cancers and normal counterparts expressed similar levels of HSC70. However, a small fraction of the breast cancers over-expressed HSC70.

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