Expression levels of haem oxygenase-1 in the omental adipose tissue and peripheral blood mononuclear cells of women with polycystic ovary syndrome

Kok Min Seow, Yu Hung Lin, Jiann Loung Hwang, Peng Hui Wang, Low Tone Ho, Yi Hsiu Lin, Chi Chang Juan

研究成果: 雜誌貢獻文章

9 引文 斯高帕斯(Scopus)

摘要

BACKGROUNDHaem oxygenase (HO)-1, an enzyme that degrades haem, plays a key role in the regulation of the inflammatory response and insulin resistance. The aim of this study was to evaluate the role of HO-1 in the regulation of insulin resistance and glucose tolerance in women with polycystic ovary syndrome (PCOS).METHODSOmental adipose tissue and human peripheral blood mononuclear cells (PBMCs) from seven women with PCOS and five healthy controls, matched for BMI and age, were analysed using western blotting and the real-time PCR.RESULTS Women with PCOS were found to have significantly higher fasting and 2-h insulin levels, a significantly higher homeostasis model assessment insulin resistance index and a lower fasting glucose-to-insulin ratio (G0/I0) than the controls. The level of HO-1 protein in omental fat (P 0.002), and the expression of HO-1 mRNA in omental fat and PBMCs from the women with PCOS were significantly lower (P 0.002 and 0.05, respectively) than those of the controls. The expression of adiponectin mRNA in omental fat was also significantly lower (P 0.02) in the women with PCOS than in the controls. However, there were no significant differences in the expression of tumour necrosis factor-α or interleukin-6 between the two groups. The level of HO-1 protein showed a significant positive correlation with the expression of HO-1 mRNA (r2 0.786, P= 0.037) and adiponectin mRNA (r2 0.7276, P
原文英語
頁(從 - 到)431-437
頁數7
期刊Human Reproduction
26
發行號2
DOIs
出版狀態已發佈 - 二月 2011

    指紋

ASJC Scopus subject areas

  • Rehabilitation
  • Obstetrics and Gynaecology
  • Reproductive Medicine

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