Expression and characterization of two STAT isoforms from Sf9 cells

Maw Sheng Yeh, Chia Hsiung Cheng, Chih Ming Chou, Ya Li Hsu, Cheng Ying Chu, Gen Der Chen, Shui Tsung Chen, Guang Chao Chen, Chang Jen Huang

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
原文英語
頁(從 - 到)814-824
頁數11
期刊Developmental and Comparative Immunology
32
發行號7
DOIs
出版狀態已發佈 - 2008

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

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